HD2C histone deacetylase and a SWI/SNF chromatin remodelling complex interact and both are involved in mediating the heat stress response in Arabidopsis
HD2C histone deacetylase and a SWI/SNF chromatin remodelling complex interact and both are involved in mediating the heat stress response in Arabidopsis
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DOI：
10.1111/pce.12756
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发表时间：
2016-10-01
影响因子：
7.3
通讯作者：
Koblowska, Marta Kamila
中科院分区：
文献类型：
--
作者：
Buszewicz, Daniel;Archacki, Rafal;Koblowska, Marta Kamila
在酵母和动物中的研究表明，组蛋白脱乙酰酶（HDAC）通常作为多蛋白复合物的组分，包括染色质重塑复合物（CRC）。然而，植物中HDAC和CRCs之间的相互作用尚未得到证实。在这里，我们提出的证据拟南芥HD 2C脱乙酰酶和BRM含有SWI/SNF CRC之间的相互作用。此外，我们揭示了HD 2C作为热应激反应调节剂的新功能。HD 2C的转录水平强烈诱导的植物进行热处理，和选择的热响应基因的表达上调热应激hd 2c突变体，表明HD 2C的行为下调热激活基因。与HD 2C的HDAC活性保持一致，HD 2C调节基因的表达改变在大多数情况下与其位点的组蛋白乙酰化增加相一致。hd 2c和brm突变体的微阵列转录组分析确定了一个共同调控的热响应基因的子集，brm hd 2c双突变对这些基因表达的影响是非加性的。此外，热处理的3周龄hd 2c，brm和brm hd 2c突变体显示出相似的生长迟缓率。总之，我们的研究结果表明，HD 2C和BRM在一个共同的遗传途径，以调节拟南芥热应激反应。
Studies in yeast and animals have revealed that histone deacetylases (HDACs) often act as components of multiprotein complexes, including chromatin remodelling complexes (CRCs). However, interactions between HDACs and CRCs in plants have yet to be demonstrated. Here, we present evidence for the interaction between Arabidopsis HD2C deacetylase and a BRM-containing SWI/SNF CRC. Moreover, we reveal a novel function of HD2C as a regulator of the heat stress response. HD2C transcript levels were strongly induced in plants subjected to heat treatment, and the expression of selected heat-responsive genes was up-regulated in heat-stressed hd2c mutant, suggesting that HD2C acts to down-regulate heat-activated genes. In keeping with the HDAC activity of HD2C, the altered expression of HD2C-regulated genes coincided in most cases with increased histone acetylation at their loci. Microarray transcriptome analysis of hd2c and brm mutants identified a subset of commonly regulated heat-responsive genes, and the effect of the brm hd2c double mutation on the expression of these genes was non-additive. Moreover, heat-treated 3-week-old hd2c, brm and brm hd2c mutants displayed similar rates of growth retardation. Taken together, our findings suggest that HD2C and BRM act in a common genetic pathway to regulate the Arabidopsis heat stress response.