Long Noncoding RNA Expression Profiling Using Arraystar LncRNA Microarrays
Long Noncoding RNA Expression Profiling Using Arraystar LncRNA Microarrays
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DOI：
10.1007/978-1-4939-3378-5_6
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Shang, Jindong
中科院分区：
文献类型：
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作者：
Shi, Yanggu;Shang, Jindong
Arraystar LncRNA微阵列设计用于在同一阵列上对LncRNA和mRNA进行全局基因表达谱分析。阵列内容以LncRNA的全面集合为特色，包括已知编码mRNA的完整集合。每个RNA转录物通过剪接点特异性探针或独特的外显子序列检测，使得可变剪接的转录物同种型或变体被可靠且准确地检测。高度优化的实验方案和高效的工作流程确保了灵敏、稳健和准确的微阵列数据生成。提供了标准数据分析用于微阵列原始数据处理、数据质量控制、基因表达聚类和热图可视化、差异表达的LncRNA和mRNA、LncRNA亚类、LncRNA与mRNA的调控关系、基因本体和途径分析。LncRNA微阵列是研究LncRNA在生物学和疾病中的有力工具，在基因表达谱、基因调控机制研究、LncRNA功能发现和生物标志物开发等方面具有广泛的应用。
Arraystar LncRNA microarrays are designed for global gene expression profiling of both LncRNAs and mRNAs on the same array. The array contents feature comprehensive collections of LncRNAs and include entire sets of known coding mRNAs. Each RNA transcript is detected by a splice junction-specific probe or a unique exon sequence, such that the alternatively spliced transcript isoforms or variants are reliably and accurately detected. The highly optimized experimental protocols and efficient workflow ensure sensitive, robust, and accurate microarray data generation. Standard data analyses are provided for microarray raw data processing, data quality control, gene expression clustering and heat map visualization, differentially expressed LncRNAs and mRNAs, LncRNA subcategories, regulatory relationships of LncRNAs with the mRNAs, gene ontology, and pathway analysis. The LncRNA microarrays are powerful tools for the study of LncRNAs in biology and disease, with broad applications in gene expression profiling, gene regulatory mechanism research, LncRNA functional discovery, and biomarker development.