Predictability of alloreactivity among unrelated individuals: role for HLA-DPB1.
Predictability of alloreactivity among unrelated individuals: role for HLA-DPB1.
复制标题
无关个体之间同种异体反应性的可预测性：HLA-DPB1 的作用。
DOI：
10.1111/j.1399-0039.1995.tb03117.x
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Yunis,EJ
中科院分区：
文献类型：
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作者：
Awdeh,ZL;Alper,CA;Fici,DA;Ronco2nd,P;Yunis,EJ
我们比较了一条染色体上携带扩展单倍型[HLA‐B8， SC01, DR3, DRB1*0301， DQB1*0201]的非亲属个体与第二条染色体上携带通用特异性HLA‐DR4的非亲属个体的混合淋巴细胞培养反应（MLR‐I）。对同一个体的基因组DNA样本进行HLA - DRB1、DQB1和DPB1等位基因的PCR分型和SSOPH分型分析，以及DOB多态性的RFLP分析。HLA - DRB1等位基因在无亲缘关系个体间的成对MLR反应中表明，HLA - DRB1匹配比HLA - DR通用型更能预测非反应性（43% vs 22%）。此外，90%的DRB1配对对有无反应性和弱反应性MLR，而只有37%的DRB1错配的非相关个体有弱反应或无反应。匹配HLA - DRB1和HLA - DPB1等位基因可消除大量具有MLR - 1反应性的细胞混合物。此外，一些DPB1错配，但不是全部，似乎不会引起MLR - 1反应性。
We compared the mixed lymphocyte culture reaction (MLR‐I) among unrelated individuals who are carriers of the extended haplotype [HLA‐B8, SC01, DR3, DRB1*0301, DQB1*0201] on one chromosome and the generic specificity HLA‐DR4 on the second chromosome. Genomic DNA samples from the same individuals were also analyzed for HLA‐DRB1, DQB1 and DPB1 alleles by PCR and SSOPH typing and for DOB polymorphism by RFLP. HLA‐DRB1 alleles, in paired MLR responses between unrelated individuals indicated that matching of HLA‐DRB1 was a better predictor of non‐reactivity than identity in HLA‐DR generic types, (43% vs 22%). Moreover, 90% of the DRB1 matched pairs had nonreactive and weakly reactive MLR, whereas only 37% of DRB1 mismatched unrelated individuals gave weak or no reactions. Matching for HLA‐DRB1 and HLA‐DPB1 alleles eliminates a significant number of cell mixtures with MLR‐I reactivity. Furthermore, some DPB1 mismatches, but not all, do not seem to elicit MLR‐I reactivity.