Cytokines Expression Induced by Ganoderma sinensis Fungal Immunomodulatory Proteins (FIP-gsi) in Mouse Spleen Cells
Cytokines Expression Induced by Ganoderma sinensis Fungal Immunomodulatory Proteins (FIP-gsi) in Mouse Spleen Cells
复制标题
灵芝真菌免疫调节蛋白（FIP-gsi）诱导小鼠脾细胞中细胞因子的表达
DOI：
10.1007/s12010-010-8916-1
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发表时间：
2010-11-01
影响因子：
3
通讯作者：
Zhou, Xuanwei
中科院分区：
文献类型：
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作者：
Li, Qizhang;Wang, Xuefei;Zhou, Xuanwei
灵芝真菌免疫调节蛋白（FIP-gsi）是FIPs家族的新成员。在克隆G.本文报道了FIP-gsi基因在大肠杆菌表达系统中的表达。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和基质辅助激光解吸/电离质谱（MALDI-MS）对重组蛋白进行分析。最后，通过诱导细胞因子基因表达来检测免疫调节活性。 FIP-gsi蛋白由111个氨基酸组成，与FIP-glu（LZ-8）的同源性为88.6%。此外，它还能提高小鼠脾细胞中白细胞介素（IL）-2、IL-3、IL-4、干扰素γ、肿瘤坏死因子α和IL-2受体（IL-2 R）的水平。
Ganoderma sinensis fungal immunomodulatory protein (FIP-gsi) was a new member of FIPs family. Based on the cloning of FIP-gsi gene from G. sinensis, this paper reported that FIP-gsi gene was expressed in Escherichia coli expression system. Then, the recombinant proteins were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Finally, the immunomodulatory activity was examined by inducing cytokine genes expression. The FIP-gsi protein was composed of 111 amino acids, and the sequence of homologous rate was 88.6% with FIP-glu (LZ-8). Furthermore, it could enhance the levels of interleukin (IL)-2, IL-3, IL-4, interferon gamma, tumor necrosis factor alpha, and IL-2 receptor (IL-2R) in mouse spleen cells.