Molecular comparison of retroviruses associated with human and simian AIDS.
Molecular comparison of retroviruses associated with human and simian AIDS.
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与人类和猿猴艾滋病相关的逆转录病毒的分子比较。
DOI：
10.1002/hon.2900030307
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发表时间：
1985
影响因子：
3.3
通讯作者：
Gardner,MB
中科院分区：
文献类型：
--
作者：
Bryant,ML;Yamamoto,J;Luciw,P;Munn,R;Marx,P;Higgins,J;Pedersen,N;Levine,A;Gardner,MB
通过电子显微镜、免疫荧光和免疫印迹技术以及病毒基因组的限制性核酸内切酶图谱，对与人类（LAV、HTLV-III、ARV）和猿猴（SAIDS-1）获得性免疫缺陷综合征相关的传染性逆转录病毒进行了比较。胞外病毒颗粒具有相似的D型形态，但胞浆内A型核仅在SAIDS病毒感染的细胞中发现。尽管三种原型艾滋病病毒的抗原相似，但未检测到与艾滋病病毒的交叉反应。分子杂交和限制性内切酶分析还表明，SAIDS 和艾滋病病毒在遗传上无关。然而，三种艾滋病病毒分离株之间只发现了与毒株多态性一致的微小差异。因此，与猕猴和人类艾滋病相关的逆转录病毒对于每个物种来说都是独特的。
Infectious retrovirus(es) associated with the human (LAV, HTLV‐III, ARV) and simian (SAIDS‐1) acquired immune deficiency syndrome were compared by electron microscopy, immunofluorescence and immunoblotting techniques and by restriction endonuclease mapping of the viral genomes. The extracellular virus particles had similar type D morphology, but intracytoplasmic type A nucleoids were found only in SAIDS virus infected cells. Although the antigens of the three prototype AIDS viruses were similar, no cross‐reactivity with the SAIDS virus was detected. Molecular hybridization and restriction enzyme analysis also revealed that the SAIDS and AIDS viruses were genetically unrelated. However, only minor differences, consistent with strain polymorphism, were found between the three AIDS virus isolates. Thus, the retroviruses associated with AIDS in macaques and humans are unique to each species.