Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle
Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle
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绵羊发情周期子宫内雌激素受体、雄激素受体和孕激素核受体的表达
DOI：
10.1111/rda.13489
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发表时间：
2019-08-01
影响因子：
1.7
通讯作者：
Luo, Wenze
中科院分区：
文献类型：
--
作者：
Duan, Hongwei;Xiao, Longfei;Luo, Wenze
雌激素、雄激素和孕激素参与子宫生理功能的调节，参与调节的蛋白质有：雌激素受体（ER）、雄激素受体（AR）和孕激素核受体（PGR）。本研究采用免疫组织化学方法检测ER α、ER β、AR和PGR在绵羊子宫中的定位。此外，我们还利用实时荧光定量PCR（RT-qPCR）和Western blot技术分析了它们在绵羊发情周期不同阶段子宫内膜和子宫肌层中的表达谱。免疫组织化学分析表明，发情期绵羊子宫中ER α、ER β、AR和PGR均存在，主要分布于子宫腔上皮、间质、腺体和子宫肌层。实时荧光定量聚合酶链反应结果显示，在子宫内膜中，ER α的表达水平在发情期最高。相反，ER β和PGR在发情前期高表达。在子宫肌层中，ER α在动情期和动情前期均高表达，ER β在动情期和间情期高表达。孕激素核受体表达在发情期最高，其次是发情后期。在子宫内膜中，ER α和ER β受体在发情前期都很丰富，而AR蛋白含量在发情期最高。在发情周期的这个阶段，子宫肌层中的PGR蛋白浓度显着低于在其他阶段观察到的。这些结果表明，这些受体是重要的绵羊生殖功能，因为它们在mRNA和蛋白质水平的表达表现出特定的时间和组织特异性的配置文件沿着发情周期。
Oestrogen, androgen and progesterone are involved in the regulation of uterine physiological functions, with the participation of the following proteins: oestrogen receptor (ER), androgen receptor (AR) and progesterone nuclear receptor (PGR). In this study, we used immunohistochemistry to detect the localization of ER alpha, ER beta, AR and PGR in sheep uterus. Additionally, we used real-time polymerase chain reaction (RT-qPCR) and Western blot technique to analyse their expression profiles at different stages of sheep oestrous cycle in the endometrium and myometrium. Immunohistochemical analysis showed that ER alpha, ER beta, AR and PGR were present in sheep uterus in oestrus, mainly in the uterine luminal epithelium, stroma, gland and myometrium. Real-time polymerase chain reaction results showed that in the endometrium, ER alpha expression level was highest in oestrus. ER beta and PGR, instead, were highly expressed in pro-oestrus. In the myometrium, ER alpha was highly expressed in both oestrus and pro-oestrus, and ER beta was highly expressed in oestrus and dioestrus. Progesterone nuclear receptor expression was highest in oestrus, followed by metoestrus. In the endometrium, both receptors ER alpha and ER beta were abundant in pro-oestrus, while the maximum AR protein content was found in oestrus. At this stage of the oestrous cycle, PGR protein concentration in the myometrium was significantly lower than those observed in other stages. These results suggest that these receptors are important for sheep reproductive function, as their expression at mRNA and protein levels exhibits particular time- and tissue-specific profiles along the oestrous cycle.