Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage.
Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage.
复制标题
基于生物活性的微球支架，含有脱细胞软骨。
DOI：
10.1002/mabi.201400472
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发表时间：
2015-07
影响因子：
4.6
通讯作者：
Detamore MS
中科院分区：
文献类型：
--
作者：
Sutherland AJ;Detamore MS
本研究的目的是制造机械功能的微球为基础的支架含有脱细胞软骨（DCC），假设这种方法将诱导大鼠骨髓间充质干细胞（rBMSCs）在体外的软骨形成。DCC来源于猪关节软骨，并使用物理和化学方法的组合脱细胞。制备四种类型的支架：仅聚（D，L-乳酸-共-乙醇酸）（PLGA）（阴性对照）、TGF-β包封（阳性对照）、用DCC涂覆的PLGA表面和DCC包封的PLGA。将这些支架与rBMSC接种并培养长达6周。在细胞接种之前，DCC涂覆的支架的压缩模量显著低于所有其他支架类型。DCC包封组和TGF-β包封组的基因表达相当。值得注意的是，在第3周，与TGF-β组相比，DCC包封的支架含有高70%的糖胺聚糖（GAG）含量和高85%的羟脯氨酸（从无细胞DCC支架中减去基线水平）。当然，生物活性在诱发DCC细胞的生物合成反应中得到证实，尽管在规定的条件下软骨形成的真实证明仍然难以捉摸。相对于用DCC涂覆，DCC的包封似乎导致改善的电池性能，尽管该发现可能是剂量依赖性观察。总的来说，通过微球为基础的支架引入DCC似乎是有前途的软骨再生的生物活性方法，虽然需要额外的研究来最终证明软骨诱导。
The aim of this study was to fabricate mechanically functional microsphere-based scaffolds containing decellularized cartilage (DCC), with the hypothesis that this approach would induce chondrogenesis of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro. The DCC was derived from porcine articular cartilage and decellularized using a combination of physical and chemical methods. Four types of scaffolds were fabricated: Poly(D,L-lactic-co-glycolic acid) (PLGA) only (negative control), TGF-β encapsulated (positive control), PLGA surface coated with DCC, and DCC-encapsulated. These scaffolds were seeded with rBMSCs and cultured up to 6 weeks. The compressive modulus of the DCC-coated scaffolds prior to cell seeding was significantly lower than all other scaffold types. Gene expression was comparable between DCC-encapsulated and TGF-β encapsulated groups. Notably, DCC-encapsulated scaffolds contained 70% higher glycosaminoglyan (GAG) content and 85% more hydroxyproline compared to the TGF-β group at week 3 (with baseline levels subtracted out from acellular DCC scaffolds). Certainly bioactivity was demonstrated in eliciting a biosynthetic response from the cells with DCC, although true demonstration of chondrogenesis remained elusive under the prescribed conditions. Encapsulation of DCC appeared to lead to improved cell performance relative to coating with DCC, although this finding may be a dose-dependent observation. Overall, DCC introduced via microsphere-based scaffolds appears to be promising as a bioactive approach to cartilage regeneration, although additional studies will be required to conclusively demonstrate chondroinductivity.