Reinvestigation of positional distribution of tetracosahexaenoic acid in triacyl-sn-glycerols of flathead flounder flesh
Reinvestigation of positional distribution of tetracosahexaenoic acid in triacyl-sn-glycerols of flathead flounder flesh
复制标题
鲽鱼肉三酰基-sn-甘油中二十四碳六烯酸位置分布的再研究
DOI：
10.1007/s12562-008-0048-y
复制
发表时间：
2009
影响因子：
1.9
通讯作者：
Y. Ando
中科院分区：
文献类型：
--
作者：
Yukihiro Tomita;Y. Ando
为了准确测定比目鱼（Hippoglossoides）三酰基甘油（TAG）中二十四碳六烯酸（24：6 n-3）在sn-1、sn-2和sn-3位的含量，对TAG中脂肪酸的位置分布进行了研究。使用适合于鱼TAG分析的程序对从三种比目鱼获得的肉TAG进行立体特异性分析。在三个位置发现24：6 n-3酸的浓度为0.3-5.5摩尔%（sn-1位置）、1.6-23.3摩尔%（sn-2位置）和0.6-8.9摩尔%（sn-3位置）。与先前的分析相反，本分析显示24：6 n-3优先在sn-2位酯化，然后是sn-3和sn-1位。其他多不饱和脂肪酸，二十二碳六烯酸（22：6 n-3; DHA）和二十二碳五烯酸（22：5 n-3; DPA），显示出类似的分布模式。这些结果表明，在海洋鱼类TAG中观察到的长链多不饱和脂肪酸的一般趋势可以扩展到24：6 n-3在平头比目鱼TAG中的分布。因为使用的比目鱼是完全为人类的食物，我们因此摄入24：6 n-3集中在sn-2位置的TAG。
Positional distribution of fatty acids in triacyl-sn-glycerols (TAG) of the flathead flounder Hippoglossoides dubius has been reinvestigated in order to accurately determine the contents of tetracosahexaenoic acid (24:6n-3) in the sn-1, sn-2, and sn-3 positions. Flesh TAG obtained from three flounders were subjected to stereospecific analysis using a suitable procedure for fish TAG analysis. The 24:6n-3 acid was found in the three positions at the concentrations of 0.3–5.5 mole% (the sn-1 position), 1.6–23.3 mole% (the sn-2 position), and 0.6–8.9 mole% (the sn-3 position). In contrast to a previous analysis, the present analysis revealed that 24:6n-3 is preferentially esterified in the sn-2 position followed by the sn-3 and sn-1 positions. Other polyunsaturated fatty acids, docosahexaenoic acid (22:6n-3; DHA) and docosapentaenoic acid (22:5n-3; DPA), showed a similar distribution pattern. These results indicate that the general tendency observed for long-chain polyunsaturated fatty acids in marine fish TAG can be extended to the distribution of 24:6n-3 in flathead flounder TAG. Because the use of flathead flounders is entirely for human food, we thus intake 24:6n-3 concentrated in the sn-2 position of their TAG.