Use of a Small Animal Radiation Research Platform (SARRP) facilitates analysis of systemic versus targeted radiation effects in the mouse ovary.
Use of a Small Animal Radiation Research Platform (SARRP) facilitates analysis of systemic versus targeted radiation effects in the mouse ovary.
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DOI：
10.1186/s13048-018-0442-8
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发表时间：
2018-08-30
影响因子：
4
通讯作者：
Duncan FE
中科院分区：
文献类型：
--
作者：
Grover AR;Kimler BF;Duncan FE
Radiation exposure is known to cause accelerated aging and damage to the ovary, but the contribution of indirect versus direct effects is not well understood. We used the Small Animal Radiation Research Platform (SARRP) (Xstrahl) to deliver radiation to precise fields equivalent to clinical practice, allowing us to investigate systemic versus targeted damage in a structure as small as the mouse ovary. The X-ray dose was kept constant at 1 Gy, but the field varied. Mice either received total body irradiation (TBI), radiation targeted to both ovaries (T2), or radiation targeted to one ovary (left) while the contralateral ovary (right) was spared (T1). Sham mice, handled similarly to the other cohorts but not exposed to radiation, served as controls. Two weeks post-exposure, ovaries were harvested and analyzed histologically to identify and count follicles within each ovary. Radiation significantly reduced primordial follicles in the TBI and T2 cohorts compared to the Sham cohort. There were no significant differences between these two irradiated groups. These findings suggest that at 1 Gy, the extent of damage to the ovary caused by radiation is similar despite the different delivery methods. When investigating the T1 cohort, targeted ovaries showed a significant decrease in primordial and growing follicles compared to non-targeted contralateral ovaries. These findings demonstrate that the SARRP is an effective strategy for delivering precise ionizing radiation to small organs such as mouse ovaries. Such tools will facilitate identifying the relative risks to ovarian function associated with different radiation fields as well as screening the efficacy of emerging fertoprotective agents. The online version of this article (10.1186/s13048-018-0442-8) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/j.1471-0528.1999.tb08180.x
发表时间：
1999-12-01
期刊：
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY
影响因子：
--
作者：
Bath, LE;Critchley, HOD;Wallace, WHB
通讯作者：
Wallace, WHB
影响因子：
3.8
作者：
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通讯作者：
Graves, Edward E.
DOI：
10.1530/rep-16-0129
发表时间：
2016-09
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Briley SM;Jasti S;McCracken JM;Hornick JE;Fegley B;Pritchard MT;Duncan FE
通讯作者：
Duncan FE
DOI：
10.1530/rep-18-0089
发表时间：
2018-06
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Kimler BF;Briley SM;Johnson BW;Armstrong AG;Jasti S;Duncan FE
通讯作者：
Duncan FE
DOI：
10.1016/0360-3016(94)00426-l
发表时间：
1995-03-30
影响因子：
7
作者：
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通讯作者：
SULLIVAN, J