Taurine reduction associated with heart dysfunction after real-world PM2.5 exposure in aged mice
Taurine reduction associated with heart dysfunction after real-world PM2.5 exposure in aged mice
复制标题
老年小鼠暴露于现实世界的 PM2.5 后牛磺酸减少与心脏功能障碍相关
DOI：
10.1016/j.scitotenv.2021.146866
复制
发表时间：
2021-04-10
影响因子：
9.8
通讯作者：
Cai, Zongwei
中科院分区：
文献类型：
--
作者：
Qi, Zenghua;Yang, Chun;Cai, Zongwei
环境PM2.5已被证明是心血管疾病的独立危险因素；然而，关于PM2.5对心血管系统的年龄相关性影响以及长期暴露后的潜在机制的信息很少。在这项研究中，通过新开发的真实环境PM2.5暴露系统，多年龄组小鼠暴露在PM2.5中，以研究长期暴露后与年龄相关的心脏效应。首先，分析了曝光系统中使用的PM2.5的化学和物理特性。记录清醒小鼠的心率，结果表明，老年小鼠暴露于PM2.5 26周后，心率显著增加。组织学分析和ELISA法检测表明，老年小鼠对PM2.5暴露在诱导心脏氧化应激和炎症方面更敏感。此外，非靶向代谢组学显示，牛磺酸与PM2.5诱导的心功能障碍有关。用LC-MS和图像质谱仪检测心脏中牛磺酸浓度的降低，可能是由于P53表达水平、ROS和炎症细胞因子的增加所致。这些结果强调了PM2.5对心血管系统的年龄依赖性效应，并提示牛磺酸可能是PM2.5引起的老年人心功能障碍的新的心脏效应靶点。(C)2021年爱思唯尔B.V.保留所有权利。
Ambient PM2.5 has been proved to be an independent risk factor for cardiovascular diseases; however, little information is available on the age-dependent effects of PM2.5 on the cardiovascular system and the underlying mechanisms following chronic exposure. In this study, multi-aged mice were exposed to PM2.5 via the newly developed real-ambient PM2.5 exposure system to investigate age-related effects on the heart after long-term exposure. First, the chemical and physical properties of PM2.5 used in the exposure system were analyzed. The heart rate of conscious mice was recorded, and results showed that exposure of aged mice to PM2.5 for 26 weeks significantly increased heart rate. Histological analysis and ELISA assays indicated that aged mice were more sensitive to PM2.5 exposure in terms of inducing cardiac oxidative stress and inflammation. Furthermore, untargeted metabolomics revealed that taurine was involved with the PM2.5-induced cardiac dysfunction. The reduced taurine concentration in the heart was examined by LC-MS and imaging mass spectrometry; it may be due to the increased p53 expression level, ROS and inflammatory cytokines. These results emphasize the age-dependent effects of PM2.5 on the cardiovascular system and suggest that taurine may be the novel cardiac effect target for PM2.5-induced heart dysfunction in the aged. (C) 2021 Elsevier B.V. All rights reserved.