Resealing dynamics of a cell membrane after electroporation.
Resealing dynamics of a cell membrane after electroporation.
复制标题
DOI：
10.1103/physreve.66.062905
复制
发表时间：
2002-12
期刊：
影响因子：
--
通讯作者：
M. Bier;Wei Chen;T. Gowrishankar;R. Astumian;Raphael C. Lee
中科院分区：
文献类型：
--
作者：
M. Bier;Wei Chen;T. Gowrishankar;R. Astumian;Raphael C. Lee
活细胞的膜由两层两极脂质分子和更大的蛋白质组成。跨膜电位在120 mV以下是生理的，耐受性良好，但当跨膜电位大于300 mV时，这种脂质双层是不稳定的。然后形成孔洞，通过这些孔洞可以发生可测量的离子流动。我们在高达440 mV的4毫秒脉冲下跟随电流通过青蛙肌肉细胞膜。我们提出了一种理论，它允许我们用膜参数来描述脉冲后电流回零的驰豫。我们得到了3.6×10(-6)N的线张力，这与人工脂双层中发现的类似。
The membrane of a living cell consists of a bilayer of amphipolar lipid molecules as well as much larger proteins. Transmembrane potentials of up to 120 mV are physiologic and well tolerated, but when the potential is more than 300 mV, this lipid bilayer is unstable. Pores are then formed through which measurable flow of ions can occur. We follow currents through frog muscle cell membranes under 4-ms pulses of up to 440 mV. We present a theory that allows us to describe the relaxation of the current back to zero after the pulse in terms of membrane parameters. We obtain a line tension of 3.6 x 10(-6) N, which is similar to that found in artificial lipid bilayers.