Dual application of beneficial microorganisms to seed during drum priming
Dual application of beneficial microorganisms to seed during drum priming
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DOI：
10.1016/j.apsoil.2007.08.001
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发表时间：
2008-01-01
影响因子：
4.8
通讯作者：
Whipps, Jolin M.
中科院分区：
文献类型：
--
作者：
Bennett, Amanda J.;Whipps, Jolin M.
首次研究了滚筒引发过程中有益微生物在胡萝卜和洋葱种子中的双重应用。一种细菌(绿化假单胞菌MA342或荧光假单胞菌CHA0)和一种真菌分离物(华支杆菌IK726d11或哈茨木霉T22)的组合在同时应用于种子时进行了配伍试验。在胡萝卜种子上，所有的微生物在引发过程中都是增殖的，而在洋葱种子上，真菌分离物的数量下降。胡萝卜和洋葱种子需要不同的接种量才能在引发过程结束时达到至少5log(10)cfu g(-1)干种子的目标接种率。温室实验表明，联合应用于种子的微生物在根和根际土壤中存活了8周，无论是单独应用于种子还是联合应用于种子，都可以看到类似的生存模式。在类似的组合中，罗氏木霉IK726d11与该细菌的组合对洋葱、胡萝卜根和根际的活性定植效果好于哈茨木霉T22。种子的初始剂量率显著影响从根际恢复的微生物数量。(C)2007 Elsevier B.V.保留所有权利。
Dual application of beneficial microorganisms to carrot and onion seed during drum priming was demonstrated for the first time. Combinations of one bacterial (Pseudomonas chlororaphis MA342 or Pseudomonas fluorescens CHA0) and one fungal isolate (Clonostachys rosea IK726d11 or Trichoderma harzianum T22) were tested for compatibility when applied simultaneously to seed. On carrot seed, all microorganisms proliferated during the priming process, whereas on onion seed the fungal isolates declined in number. Different inoculum rates were required for carrot and onion seed to achieve the target rate of at least 5 log(10) cfu g(-1) dry seed at the end of the priming process. Glasshouse experiments showed that the microorganisms applied in combination to the seed survived on roots and in rhizosphere soil for 8 weeks, with similar survival patterns seen irrespective of whether the microorganisms were applied to seed singly or in combination. C. rosea IK726d11 in combination with the bacteria actively colonised the onion and carrot roots and rhizosphere better than T. harzianum T22 in similar combinations. Initial dose rates on the seed significantly affected the numbers of microorganisms recovered from the rhizosphere. (c) 2007 Elsevier B.V. All rights reserved.