Extra- and intracellular signaling pathways regulating nodule formation by hemocytes of the silkworm, Bombyx mori(Lepidoptera: Bombicidae)
Extra- and intracellular signaling pathways regulating nodule formation by hemocytes of the silkworm, Bombyx mori(Lepidoptera: Bombicidae)
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调节家蚕血细胞结节形成的胞外和胞内信号通路（鳞翅目：Bombicidae）
DOI：
10.11416/jibs.80.2_049
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发表时间：
2011
影响因子：
--
通讯作者：
R. Sato
中科院分区：
文献类型：
--
作者：
Asahi Suzuki;Yasutaka Yoshizawa;Shiho Tanaka;Madoka Kitami;R. Sato
家蚕能在30分钟内清除血淋巴中的106个细菌细胞，其早期防御机制主要依赖于双糖识别结构域（CRD）C型凝集素介导的病原识别和血细胞形成的结节。为了理解这一现象，本研究探讨了与B血细胞结节形成相关的细胞间和细胞内信号转导途径。森。C-型凝集素抑制剂二硫苏糖醇和乙二胺四乙酸抑制体内结节形成，和一个双CRD的C-型凝集素需要在体外结节样聚集。这些结果表明，双CRD的C型凝集素需要作为病原体识别受体的结节形成。丝氨酸蛋白酶抑制剂，如盐酸苄脒，抑肽酶，亮抑酶肽抑制结节的形成，凝血酶，丝氨酸蛋白酶，诱导体内结节样聚集。这表明丝氨酸蛋白酶在导致结节形成的细胞外信号级联中充当介体。磷脂酶C的抑制剂，U-73122，抑制体内结节形成和体外结节样聚集。EDTA和百日咳毒素抑制体内结节形成。这些结果表明，由两个G蛋白介导的信号转导通路触发的细胞反应在结节形成中起着重要作用。
It has been suggested that early defense mechanisms of the silkworm Bombyx mori, which can clear 106 bacterial cells from the hemolymph within 30 minutes, largely depend on pathogen recognition mediated by dualcarbohydrate recognition domain (CRD) C-type lectins and nodule formation by hemocytes. In order to understand this phenomenon, the present study explores both inter-and intracellular signal transduction pathways in association with nodule formation by hemocytes of B. mori. The C-type lectin inhibitors dithiothreitol and ethylenediaminetetraacetic acid inhibited in vivo nodule formation, and a dual-CRD C-type lectin was required for in vitro nodule-like aggregation. These results suggested that dual-CRD C-type lectins were required as pathogen-recognition receptors for nodule formation. Serine protease inhibitors such as benzamidine hydrochloride, aprotinin, and leupeptin inhibited nodule formation, and thrombin, a serine protease, induced in vivo nodule-like aggregation. This indicates that serine proteases behave as mediators in the extracellular signal cascade leading to nodule formation. An inhibitor of phospholipase C, U-73122, inhibited both in vivo nodule formation and in vitro nodule-like aggregation. EDTA and pertussis toxin inhibited in vivo nodule formation. These results suggest that the cell reaction triggered by the two G protein-mediated signal transduction pathways plays a significant role in nodule formation.
影响因子：
3.8
作者：
Yu, XQ;Jiang, HB;Kanost, MR
通讯作者：
Kanost, MR
影响因子：
2.9
作者：
Yu, XQ;Kanost, MR
通讯作者：
Kanost, MR