Rapid analysis of cholesterol-elevating compounds in coffee brews by off-line high-performance liquid chromatography high-resolution gas chromatography
Rapid analysis of cholesterol-elevating compounds in coffee brews by off-line high-performance liquid chromatography high-resolution gas chromatography
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DOI：
10.1021/jf980934v
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发表时间：
1999-04-01
影响因子：
6.1
通讯作者：
Blanch, GP
中科院分区：
文献类型：
--
作者：
del Castillo, MLR;Herraiz, M;Blanch, GP
提出了一种新的方法，可以快速、可靠地测定不同咖啡冲泡中升高胆固醇的咖啡醇和咖啡豆醇的含量。该方法包括通过高效液相色谱法对样品进行预分离，收集选定的馏分，然后通过高分辨率气相色谱法对其进行后续分析，使用在分裂模式下操作的程控温度汽化器作为采样系统。在此条件下，咖啡醇和咖啡豆醇的回收率分别高达87%和94%，检出限分别为0.06 ppm和0.04 ppm。举例比较了用不同的冲泡方法从同一种磨碎的烘焙咖啡中得到的咖啡醇和咖啡豆醇的含量，结果表明用咖啡袋冲泡的咖啡醇和咖啡豆醇的含量最低。
A new method is proposed to analyze the cholesterol-elevating cafestol and kahweol which allows their rapid and reliable determination in different coffee brews. The method involves the preseparation of the sample by high-performance liquid chromatography, the collection of the selected fraction, and its subsequent analysis by high-resolution gas chromatography using a programmed temperature vaporizer operated in the split mode as sampling system. Under the experimental conditions investigated, recoveries as high as 87% (cafestol) and 94% (kahweol) were achieved while detection Limits equal to 0.06 and 0.04 ppm for cafestol and kahweol, respectively, were obtained. Examples are given comparing levels of cafestol and kahweol resulting from the same ground roasted coffee by different brewing methods, which show the lowest values for brews prepared from coffee bags.