The nature of the ESR signal in lyophilized tissue and its relevance to malignancy.
The nature of the ESR signal in lyophilized tissue and its relevance to malignancy.
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DOI：
10.1038/bjc.1984.10
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发表时间：
1984-01
影响因子：
8.8
通讯作者：
Swartz, H M
中科院分区：
文献类型：
--
作者：
Dodd, N J;Swartz, H M
从冷冻和冻干组织中获得的9 GHz和35 GHz光谱与含有抗坏血酸的模型体系的光谱相比较，证实了组织的“冷冻干燥信号”的主要成分是抗坏血酸自由基，通过在惰性基质上的吸附而稳定。缺氧条件下信号的大小被证明是细胞损伤的衡量标准，这允许细胞内的抗坏血酸被氧化。当冷冻干燥样品暴露在空气中时，由于可用组织抗坏血酸的自氧化，信号增加。在潮湿的大气条件下，抗坏血酸自由基很容易腐烂，留下其他自由基，这些自由基似乎是由抗坏血酸或抗坏血酸自由基与某些组织成分相互作用形成的。结果表明，尽管研究广泛，但冻干组织的自由基ESR信号并不是肿瘤独有的，与肿瘤的恶性程度无关。
Comparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.