DETERMINATION OF WATER PERMEABILITY COEFFICIENT AND ITS ACTIVATION-ENERGY FOR RABBIT SPERMATOZOA
DETERMINATION OF WATER PERMEABILITY COEFFICIENT AND ITS ACTIVATION-ENERGY FOR RABBIT SPERMATOZOA
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DOI：
10.1006/cryo.1995.1016
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发表时间：
1995-04-01
期刊：
影响因子：
2.7
通讯作者：
WATSON, PF
中科院分区：
文献类型：
--
作者：
CURRY, MR;REDDING, BJ;WATSON, PF
兔精子的临界张力（50%的细胞膨胀超过其最大体积-表面积比并溶解的张力）在22 ℃下测量为45.6 mOsm。为了确定温度对临界张力的影响，将细胞平衡至15、25和35 ° C，并分别测量临界张力为52.9、42.2和32.4mOsm。通过将细胞暴露于蒸馏水的时间增加，测量家兔精子在这些温度下实现溶解所需的时间。根据这些结果，计算了家兔精子对水的渗透系数（L（p））及其活化能（E（a））。在25 ℃时，L（p）为0.63 μ m/min/atm，明显与温度有关; E（a）为17.8 kcal/mol。兔精子似乎具有低L（p）和高E（a），与迄今为止检查的所有其他物种的精子所观察到的具有高L（p）和低E（a）的情况相反。尽管如此，所获得的值允许在冷冻保存过程中细胞存活的冷却速率建模，以保持通常采用的冷却速率。(C)出版社：Academic Press
Critical tonicity (tonicity at which 50% of cells swell beyond their maximum volume-to-surface area ratio and lyse) of rabbit spermatozoa was measured as 45.6 mOsm at 22 degrees C. To determine the temperature effect on critical tonicity, cells were equilibrated to 15, 25, and 35 degrees C and critical tonicity was measured as 52.9, 42.2, and 32.4 mOsm, respectively. The time taken for rabbit sperm to achieve lysis at these temperatures was measured by exposing cells for increasing times to distilled water. From these results values for the permeability coefficient of rabbit spermatozoa to water (L(p)) and its activation energy (E(a)) were calculated. At 25 degrees C, L(p) was 0.63 mu m/min/atm and was clearly temperature dependent; E(a) was 17.8 kcal/mol. Rabbit spermatozoa appear to have a low L(p) and high E(a), the opposite of the situation seen with spermatozoa from all other species examined to date which have high L(p) and low E(a). Nevertheless, the values obtained permit modeling of cooling rates for cell survival during cryopreservation in keeping with cooling rates commonly employed. (C) 1995 Academic Press, Inc.