Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives
Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives
复制标题
对吩噻嗪衍生物敏感的大肠杆菌 dnaA 突变体的表型
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
K. Sekimizu
中科院分区：
文献类型：
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作者：
T. Mizushima;T. Shinpuku;H. Katayama;K. Kataoka;Lei Guo;T. Miki;K. Sekimizu
摘要氟奋乃静在体外可抑制心磷脂对DNAA蛋白的激活。因此，我们检测了大肠杆菌的温度敏感型Dna A突变体对氟奋乃静和其他吩噻嗪衍生物的敏感性。在所测试的8个Dna A突变体中，dna A5、dna A46、dna A602和dna A604突变体对吩噻嗪衍生物的敏感性高于野生型菌株。在DNAA蛋白N端区发生突变的dna A508和dna A167突变体对吩噻嗪类化合物也表现出更高的敏感性。另一方面，在DNAA蛋白的C末端区域有损伤的dna A204和dna A205突变体对吩噻嗪衍生物表现出与野生型菌株相同的敏感性。与含有野生型Dna A基因的质粒的互补分析和噬菌体P1介导的转导证实了Dna A突变是导致这些敏感表型的原因。
Abstract The activation of DnaA protein by cardiolipin is inhibited by fluphenazine in vitro. We therefore examined the sensitivity of temperature-sensitive dnaA mutants of Escherichia coli to fluphenazine and other phenothiazine derivatives. Among the eight dnaA mutants tested, dnaA5, dnaA46 dnaA602, and dnaA604, mutants with mutations in the putative ATP binding site of DnaA protein, showed higher sensitivities to phenothiazine derivatives than did the wild-type strain. The dnaA508 and dnaA167 mutants, which have mutations in the N-terminal region of DnaA protein, also showed higher sensitivities to phenothiazine derivatives. On the other hand, the dnaA204 and dnaA205 mutants, with lesions in the C-terminal region of the DnaA protein, showed the same sensitivity to phenothiazine derivatives as the wild-type strain. Complementation analysis with a plasmid containing the wild-type dnaA gene and phage P1-mediated transduction confirmed that dnaA mutations are responsible for these sensitivity phenotypes.
膜附着激活 dnaA 蛋白，这是大肠杆菌中染色体复制的起始蛋白。
DOI：
10.1073/pnas.85.19.7202
发表时间：
1988
影响因子：
11.1
作者：
Yung,BY;Kornberg,A
通讯作者：
Kornberg,A