In vitro studies on the effect of yolk sac antisera on functions of the visceral yolk sac: I. Pinocytosis and transport of small molecules.
In vitro studies on the effect of yolk sac antisera on functions of the visceral yolk sac: I. Pinocytosis and transport of small molecules.
复制标题
卵黄囊抗血清对内脏卵黄囊功能影响的体外研究：一、胞饮作用和小分子转运。
DOI：
10.1002/tera.1420340313
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Brent,RL
中科院分区：
文献类型：
--
作者：
Lerman,S;Koszalka,TR;Jensen,M;Andrew,CL;Beckman,DA;Brent,RL
许多实验室都曾报道过妊娠动物使用致畸性抗血清会产生先天畸形。这项工作集中了我们的注意力在卵黄囊胎盘的重要性，支持大鼠胚胎早期器官发生和卵黄囊功能障碍的意义在啮齿动物致畸。本文报道的研究涉及致畸抗血清对卵黄囊转运过程的影响;特别是胞饮作用（通过14 C-蔗糖摄取测量）和利用14 C-α-氨基异丁酸（AIB）和3 H-2-脱氧葡萄糖（DOG）的小分子转运。我们试图确定是否有几种不同的卵黄囊定位抗体干扰这些运输过程，如果是这样的话，哪些运输过程是最受影响的。实验结果表明，致畸抗血清干扰的过程中的卵黄囊胞饮和胞饮可以减少多达40%。非致畸性抗血清，即使当它们定位在卵黄囊，不干扰的过程中的胞饮。此外，致畸抗血清不干扰卵黄囊中小分子（AIB或DOG）的转运。这些结果表明，虽然卵黄囊中抗血清的荧光定位并不总是表明致畸性的潜力，但胞饮作用的减少可能与致畸和胚胎病事件直接相关。这项工作重申了卵黄囊在啮齿动物器官发生过程中的重要性，以及卵黄囊功能障碍在先天性畸形的发展中起重要作用。来自用分离的卵黄囊进行的内吞实验的结果确实鉴定了胚胎毒性抗血清（即，全内脏卵黄囊抗血清），因此该方法可用作这些抗血清的快速生物测定。内吞指数的简单测量作为一个标志物，用于确定致畸性的未表征的血清将代表一个相当大的节省时间，精力和动物。
The production of congential malformations by the administration of teratogenic antisera to pregnant animals has been reported from many laboratories. This work has focused our attention on the importance of the yolk sac placenta in supporting the rat embryo during early organogenesis and the significance of yolk sac dysfunction in rodent teratogenesis. The studies reported in this article deal with the effect of teratogenic antisera on the process of yolk sac transport; specifically pinocytosis (as measured by14C‐sucrose uptake) and small‐molecule transport utilizing14C‐α‐aminoisobutyric acid (AIB) and3H‐2‐deoxyglucose (DOG). We sought to determine whether several different yolk sac localizing antibodies interfere with these transport processes, and, if so, which transport processes were most affected.The results of the experiments indicated that teratogenic antisera interfered with the process of pinocytosis in the yolk sac and that pinocytosis can be reduced as much as 40%. Nonteratogenic antisera, even when they localized in the yolk sac, did not interfere with the process of pinocytosis. Furthermore, the teratogenic antisera did not interfere with the transport of small molecules (either AIB or DOG) in the yolk sac. These results indicated that while fluorescent localization of an antiserum in the yolk sac did not invariably indicate the potential for teratogenicity, it is likely that the reduction in pinocytosis may directly correlate with the teratologic and embryopathic events. This work reaffirms the view that the yolk sac in important during rodent organogenesis and that yolk sac dysfunction can play an important role in the development of congenital malformations. The results from the endocytosis experiments with explanted yolk sacs did identify the embryotoxic antiserum (i.e., whole visceral yolk sac antiserum) and thus this method may have utility as a rapid bioassay for these antisera. The simple measurement of endocytic index as a marker for the determination of teratogenicity of uncharacterized sera would represent a considerable saving in time, effort, and animals.