Donor plasma mitochondrial DNA is associated with antibody-mediated rejection in renal allograft recipients.
Donor plasma mitochondrial DNA is associated with antibody-mediated rejection in renal allograft recipients.
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供体血浆线粒体 DNA 与同种异体肾移植受者抗体介导的排斥反应相关
DOI：
10.18632/aging.202654
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发表时间：
2021-03-10
期刊：
影响因子：
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通讯作者：
Sun Q
中科院分区：
文献类型：
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作者：
Han F;Sun Q;Huang Z;Li H;Ma M;Liao T;Luo Z;Zheng L;Zhang N;Chen N;Hong L;Na N;Sun Q
我们先前研究表明供体血浆线粒体DNA（dmtDNA）水平与移植肾功能相关。本研究的目的是确定dmtDNA水平是否与抗体介导的排斥反应（ABMR）的发生有关。这是一项回顾性开放队列研究，包括2015年1月至2017年12月入组的167名供体和323名受体。我们使用定量实时聚合酶链反应定量供体血浆中存在的mtDNA水平。急性排斥组血浆dmtDNA水平明显高于对照组（0.156比0.075，P<0.001）。多因素Logistic回归分析显示dmtDNA水平与AR也有显著相关性（OR=1.588，95% CI 1.337-4.561，p<0.001）。当dmtDNA水平>0.156时，AR的发生率为62.9%。ABMR组血浆dmtDNA水平显著高于T细胞介导的排斥组（0.185对0.099，p=0.032）。dmtDNA预测ABMR的受试者工作特征曲线下面积高达0.910（95% CI 0.843-0.977）。我们证明血浆dmtDNA是ABMR的独立危险因素，这在器官评估中是有价值的。dmtDNA水平可能是ABMR的第一个预测指标。
We previously showed that donor plasma mitochondrial DNA (dmtDNA) levels were correlated with renal allograft function. The aim of the current study was to determine whether dmtDNA levels are associated with the occurrence of antibody-mediated rejection (ABMR). This is a retrospective open cohort study comprised of 167 donors and 323 recipients enrolled from January 2015 to December 2017. We quantified the mtDNA level present in donor plasma using quantitative real-time polymerase chain reaction. The average plasma dmtDNA level in the acute rejection (AR) group was higher than that of the control group (0.156 versus 0.075, p<0.001). Multivariate logistic regression analysis showed that dmtDNA levels were also significantly associated with AR (OR=1.588, 95% CI 1.337-4.561, p<0.001). When the dmtDNA level was >0.156, the probability of AR was 62.9%. The plasma dmtDNA level in the ABMR group was significantly higher than that of the T cell-mediated rejection group (0.185 versus 0.099, p=0.032). The area under the receiver operating characteristic curve of dmtDNA for prediction of ABMR was as high as 0.910 (95% CI 0.843-0.977). We demonstrated that plasma dmtDNA was an independent risk factor for ABMR, which is valuable in organ evaluation. dmtDNA level is a possible first predictive marker for ABMR.