Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component
Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component
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两个剪接的 1 型单纯疱疹病毒立即早期 mRNA 的结构，映射在病毒 DNA S 成分的独特区域和重复区域的连接处
DOI：
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发表时间：
1981
影响因子：
5.4
通讯作者：
G. V. Vande Woude
中科院分区：
文献类型：
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作者：
R. Watson;M. Sullivan;G. V. Vande Woude
我们研究了两个单纯疱疹病毒1型即刻早期(IE)RNA(IE mRNA-4和IE mRNA-5)的结构，它们映射在病毒DNA短成分的独特区域(US)和重复区域(TRS/IR)的交界处。将IE胞质RNA与单纯疱疹病毒1型DNA限制性内切酶片段杂交，用单链特异性核酸酶S1和外切酶VII双酶切，琼脂糖凝胶电泳法分析产物。用核酸酶消化技术获得的数据得到了由多腺化IE RNA和病毒DNA片段形成的R环结构的电子显微镜证实。结果发现，IE mRNA4和IEmRNA5均含有一个260碱基的5‘端共转录产物，并定位在TRS/IRS的等位基因上。这些5‘端序列被剪接到1,450个碱基(对于IE mRNA-4)和1,540个碱基(对于IE mRNA-5)的3’端共转录产物上。3‘端共转录本含有TRs/IRS和我们两端编码的序列，表明IE m RNA-4和IE m RNA-5基因所含的内含子完全位于重复序列内，长度约为150个碱基对。这些数据表明，这些基因可能包含共同和独特的成分，并讨论了该模型的含义。
We have examined the structures of two herpes simplex virus type 1 immediate-early (IE) RNAs (IE mRNA-4 and IE mRNA-5) which map at the junctions of the unique (Us) and reiterated regions (TRs/IRs) of the virus DNA short component. Hybrids between IE cytoplasmic RNA and herpes simplex virus type 1 DNA restriction fragments were digested with single-strand-specific nucleases S1 and exonuclease VII, and the products were analyzed by agarose gel electrophoresis. Data obtained with the nuclease digestion technique were confirmed by electron microscopy of R-loop structures formed with polyadenylated IE RNA and virus DNA fragments. It was found that both IE mRNA-4 and IE mRNA-5 contained a 260-base 5'-terminal cotranscript which mapped at equivalent loci within TRs/IRs. These 5'-terminal sequences were shown to be spliced to 3'-terminal cotranscripts of 1,450 bases (for IE mRNA-4) and 1,540 bases (for IE mRNA-5). The 3'-terminal cotranscripts contained sequences encoded by both TRs/IRs and opposite ends of Us, indicating that the introns contained by the IE mRNA-4 and IE mRNA-5 genes, found to be approximately 150 base pairs in size, mapped entirely within the reiterated sequences. The data suggest that these genes may contain common and unique components, and the implications of this model are discussed.