Design principles of a minimal auxin response system
Design principles of a minimal auxin response system
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DOI：
10.1038/s41477-020-0662-y
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发表时间：
2020-05-01
期刊：
影响因子：
18
通讯作者：
Weijers, Dolf
中科院分区：
文献类型：
--
作者：
Kato, Hirotaka;Mutte, Sumanth K.;Weijers, Dolf
生长素通过调节ARF转录因子活性的基因表达系统控制陆地植物的许多生长过程（1-3）。在大多数陆地植物中，编码生长素反应组分的家族中的基因重复产生了巨大的复杂性，并且新功能化使得在发育过程中能够产生各种独特的反应输出（1，3，4）。然而，目前还不清楚是什么基本的生物化学原理构成了这个复杂的反应系统。通过研究地钱中的最小系统，我们得到了一个直观而简单的模型，其中一个单一的生长素依赖的A-ARF激活基因表达。它被抑制共同靶基因的生长素非依赖性B-ARF拮抗。这两种ARF蛋白的表达模式定义发育区，其中生长素反应是允许的，定量调整或防止。这种基本的设计可能代表了祖先的系统，并形成了膨胀的基础上，复杂systems.The最小的监管逻辑和生长素信号通路的进化历史进行了探讨，通过研究更简单的生长素网络地钱与遗传相结合，域改组和生化策略。
Auxin controls numerous growth processes in land plants through a gene expression system that modulates ARF transcription factor activity(1-3). Gene duplications in families encoding auxin response components have generated tremendous complexity in most land plants, and neofunctionalization enabled various unique response outputs during development(1,3,4). However, it is unclear what fundamental biochemical principles underlie this complex response system. By studying the minimal system in Marchantia polymorpha, we derive an intuitive and simple model where a single auxin-dependent A-ARF activates gene expression. It is antagonized by an auxin-independent B-ARF that represses common target genes. The expression patterns of both ARF proteins define developmental zones where auxin response is permitted, quantitatively tuned or prevented. This fundamental design probably represents the ancestral system and formed the basis for inflated, complex systems.The minimal regulatory logic and the evolutionary history of the auxin signalling pathway are explored by studying the much simpler auxin network in Marchantia with a combination of genetic, domain shuffling and biochemical strategies.