Nerve growth factor enhances expression of neuron-glia cell adhesion molecule in PC12 cells.
Nerve growth factor enhances expression of neuron-glia cell adhesion molecule in PC12 cells.
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DOI：
10.1083/jcb.102.2.413
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发表时间：
1986-02
期刊：
影响因子：
--
通讯作者：
Edelman GM
中科院分区：
文献类型：
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作者：
Friedlander DR;Grumet M;Edelman GM
神经元-胶质细胞黏附分子(Ng-CAM)在哺乳动物脑组织和PC12嗜铬细胞瘤细胞中分别被鉴定为MR 200,000和MR 230,000种。当神经生长因子(NGF)处理PC12细胞时，细胞表面Ng-CAM的数量增加了近3倍，而神经细胞黏附分子(N-CAM)的数量没有变化。NGF诱导的大分子外膜糖蛋白(NILE)在经NGF处理的PC12细胞中表达增强，已被鉴定。Ng-CAM和Nile在PC12细胞中的相对分子质量、发育过程中的表达以及对NGF的反应性相似，提示这两个分子是相关的。此外，Ng-CAM和Nile的抗体发生交叉反应，在有限的蛋白降解后，两个分子具有相似的肽图。此外，Ng-CAM的抗体抑制了神经突起的聚集，这是尼罗河流域共有的一种功能特性。结果表明，细胞黏附分子可以选择性地对生长因子做出反应，这表明尼罗河实际上是哺乳动物的Ng-CAM。
The neuron-glia cell adhesion molecule (Ng-CAM) has been identified in mammalian brain tissue and PC12 pheochromocytoma cells as Mr 200,000 and Mr 230,000 species, respectively. When PC12 cells were treated with nerve growth factor (NGF), the amount of Ng-CAM at the cell surface was increased approximately threefold, whereas the amount of the neural cell adhesion molecule (N-CAM) remained unchanged. An NGF-inducible large external glycoprotein (NILE) has been previously identified by its enhanced expression in NGF-treated PC12 cells. Ng-CAM and NILE are similar in molecular weight, expression during development, and responsiveness to NGF in PC12 cells, suggesting that the two molecules are related. In addition, antibodies to Ng-CAM and NILE cross-reacted and the molecules had similar peptide maps after limited proteolysis. Moreover, antibodies to Ng-CAM inhibited fasciculation of neurites, a functional property shared with NILE. The results show that cell adhesion molecules can respond selectively to growth factors and suggest that NILE is, in fact, mammalian Ng-CAM.