Identification of early neurogenic cells in the neural crest lineage.
Identification of early neurogenic cells in the neural crest lineage.
复制标题
神经嵴谱系中早期神经源性细胞的鉴定。
DOI：
10.1016/0012-1606(92)90285-o
复制
发表时间：
1992
影响因子：
2.7
通讯作者：
Weston,JA
中科院分区：
文献类型：
--
作者：
Marusich,MF;Weston,JA
多能神经嵴细胞在发育过程中逐渐受到限制。这种限制的顺序和在早期发育中对嵴源细胞施加这种限制的时间在很大程度上是未知的。我们已经使用了人类自身抗体（抗胡）来表征鸟类神经嵴衍生细胞的神经源性群体。抗-Hu特异性结合于较老（>E4）鹌鹑胚胎中的神经元和神经内分泌细胞。在发育早期，抗-Hu还结合缺乏神经元形态且不表达其他神经元性状的神经嵴衍生细胞亚群。这些细胞可能代表了早期嵴细胞谱系中一个假定的神经原性前体亚群。为了验证这一假设，我们已经确定了已知具有或缺乏产生新神经元的能力的嵴源性群体内的抗Hu免疫反应性。我们报告，抗胡+非神经元细胞的存在与给定细胞群的神经发生能力相关。此外，抗Hu+非神经元细胞是短暂的，似乎被抗Hu+神经元细胞所取代。我们的结论是，抗胡是一个非常早期的指标之间的嵴源性细胞的神经发生和抗胡+非神经元细胞是神经前体或不成熟的神经元。
Pluripotent neural crest cells are restricted progressively during development. Thesequenceof restrictions and the time(s) in early development at which such restrictions are imposed on crest-derived cells are largely unknown. We have used a human autoantibody (Anti-Hu) to characterize neurogenic populations of avian neural crest-derived cells. Anti-Hu binds specifically to neurons and neuroendocrine cells in older (>E4) quail embryos. Early in development, Anti-Hu also binds a subpopulation of neural crest-derived cells that lack neuronal morphology and do not express other neuronal traits. These cells may represent a putative neurogenic precusor subpopulation within the early crest cell lineage. To test this hypothesis, we have characterized Anti-Hu immunoreactivity within crest-derived populations known to have, or to lack, the ability to give rise to new neurons. We report that the presence of Anti-Hu+nonneuronal cells is correlated with the neurogenic ability of a given cell population. Moreover, Anti-Hu+nonneuronal cells are transient and appear to be replaced by Anti-Hu+neuronal cells. We conclude that Anti-Hu is a very early indicator of neurogenesis among crest-derived cells and that Anti-Hu+nonneuronal cells are either neurogenic precursors or immature neurons.