Three dimensional MEMS microfluidic perfusion system for thick brain slice cultures
Three dimensional MEMS microfluidic perfusion system for thick brain slice cultures
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DOI：
10.1007/s10544-006-9004-8
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发表时间：
2007-02-01
影响因子：
2.8
通讯作者：
Allen, Mark G.
中科院分区：
文献类型：
--
作者：
Choi, Yoonsu;McClain, Maxine A.;Allen, Mark G.
体外组织培养模型通常以它们在体内复制功能的能力为基准。体外系统的局限性之一是难以保存精心编排的细胞群体，特别是在生成三维组织等价物方面。例如，组织工程应用涉及大型高密度结构，需要一个能够将足够的氧气和营养物质应用于组织内部区域的灌流系统。对于用于体外培养的厚组织切片而言尤其如此。我们以SU-8光敏环氧树脂为原料，通过适当的后处理，制作了一种可用于高密度体外组织培养的微针灌流装置。该设备在采集的脑组织切片中测试了其提高生存能力的能力。之所以选择这种模式，是因为它对营养供应的中断非常敏感。通过活体-死体区分荧光染色和共聚焦显微镜评估，短期内存活率明显提高。这种灌流系统为神经生物学和其他培养系统开辟了许多可能性。
In vitro tissue culture models are often benchmarked by their ability to replicate in vivo function. One of the limitations of in vitro systems is the difficulty in preserving an orchestrated cell population, especially for generating three-dimensional tissue equivalents. For example, tissue-engineering applications involve large high-density constructs, requiring a perfusing system that is able to apply adequate oxygen and nutrients to the interior region of the tissue. This is particularly true with respect to thick tissue sections harvested for in vitro culture. We have fabricated a microneedle-based perfusion device for high-cell-density in vitro tissue culture from SU-8 photosensitive epoxy and suitable post-processing. The device was tested for its ability to improve viability in slices of harvested brain tissue. This model was chosen due to its acute sensitivity to disruptions in its nutrient supply. Improved viability was visible in the short term as assessed via live-dead discriminating fluorescent staining and confocal microscopy. This perfusion system opens up many possibilities for both neurobiological as well as other culture systems.