The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem cells
The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem cells
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DOI：
10.1089/bio.2019.0103
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发表时间：
2020-08-17
影响因子：
1.6
通讯作者：
Acker, Jason P.
中科院分区：
文献类型：
--
作者：
Borzou, Bahareh;Mehrabani, Davood;Acker, Jason P.
目的：比较人羊水干细胞（hAFSCs）在不同年龄母体和AmnioMAX和Dulbecco改良Eagle’s培养基（DMEM）中的生长动力学。材料与方法：对16例妊娠16 ~ 18周行羊膜穿刺术的孕妇提供3毫升羊水（AF）。将母亲分为20-29岁（n = 5）、30-39岁（n = 5）和40-49岁（n = 6）三个年龄组。立即离心AF，培养细胞颗粒。细胞形态学表征，流式细胞术和成骨和脂肪诱导。测定了种群倍增时间（PDT）和生长动力学。AmnioMAX中培养的AFSCs在不同年龄组中进行比较。比较了40 ~ 49岁孕妇在AmnioMAX和DMEM中培养AFSCs的生长动力学。结果：AFSCs粘附在培养瓶上，呈纺锤形，成骨和脂肪诱导阳性，CD73、CD90和CD105标记表达阳性，CD34和CD45标记表达阴性。20-29岁、30-39岁和40-49岁女性PDT分别为30.9、38.3和43.9小时，年龄越小细胞增殖越高。对比AmnioMAX和DMEM， PDT分别为53和96.9小时，表明AmnioMAX的细胞增殖能力更高。结论：在AmnioMAX和年轻母亲样本中，hAFSCs具有较高的增殖和可塑性。这些发现可以添加到文献中，并为再生医学开辟了一条新的途径，当hAFSCs成为细胞治疗的目标时。
Aim:This study compared growth kinetics of human amniotic fluid stem cells (hAFSCs) in different maternal age groups and two different media of AmnioMAX and Dulbecco's modified Eagle's medium (DMEM). Materials and Methods:Three milliliters of amniotic fluid (AF) was provided from 16 pregnant women who were referred for amniocentesis from 16 to 18 weeks of gestation. Mothers were divided to 20-29 (n = 5), 30-39 (n = 5) and 40-49 (n = 6) years old age groups. AF was immediately centrifuged and the cell pellet was cultured. Cells were characterized morphologically, by flow cytometry and for osteogenic and adipogenic inductions. Population doubling time (PDT) and growth kinetics were determined. AFSCs cultured in AmnioMAX were compared in various age groups. A comparison of growth kinetics of AFSCs cultured in AmnioMAX and DMEM from 40 to 49 years old pregnant women was undertaken. Results:AFSCs were adherent to culture flasks and were spindle shape, and positive for osteogenic and adipogenic inductions and for expression of CD73, CD90 and CD105 markers, but negative for CD34 and CD45. PDT among 20-29, 30-39, and 40-49 years old women was 30.9, 38.3, and 43.9 hours, respectively showing a higher cell proliferation in younger ages. When comparing AmnioMAX and DMEM, PDT was 53 and 96.9 hours, respectively denoting to a higher cell proliferation in AmnioMAX. Conclusions:Higher proliferation and plasticity of hAFSCs were noted in AmnioMAX and in younger mothers' samples. These findings can be added to the literature and open a new avenue in regenerative medicine, when hAFSCs are targeted for cell therapy purposes.