Experimental investigation of effect of ultrasonic duty cycle on generation of reactive oxygen species for highly efficient sonodynamic treatment
Experimental investigation of effect of ultrasonic duty cycle on generation of reactive oxygen species for highly efficient sonodynamic treatment
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DOI：
10.35848/1347-4065/ab82a5
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发表时间：
2020-04
影响因子：
1.5
通讯作者：
Kenk Tsukahara;S. Umemura;S. Yoshizawa
中科院分区：
文献类型：
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作者：
Kenk Tsukahara;S. Umemura;S. Yoshizawa
声动力治疗（SDT）是一种结合超声、活性氧（ROS）和声敏剂的非侵入性癌症治疗方法。超声波产生的空化气泡振荡和坍缩时，由于气泡内部的高温高压产生ROS。通过在靶组织中产生空化泡，可以通过活性氧的细胞毒性进行治疗，这种细胞毒性可以通过声敏剂进一步增强。在SDT中，单次HIFU治疗区域小，导致治疗时间长，需要通过提高ROS生成效率来缩短治疗时间。通过鲁米诺的声化学发光可以观察到活性氧。在本研究中，聚丙烯酰胺凝胶浸泡了鲁米诺溶液作为超声暴露的目标。通过捕获声化学发光发射和空化气泡的高速摄影，研究了HIFU暴露占空比对ROS产生的影响。
Sonodynamic treatment (SDT) is a non-invasive treatment of cancer, combining ultrasound, reactive oxygen species (ROS), and sonosensitizer. When cavitation bubbles generated by ultrasound oscillate and collapse, ROS are generated due to the high temperature and high pressure inside the bubble. By generating cavitation bubbles in a target tissue, it can be treated by the cytotoxicity of ROS, which can be further enhanced by a sonosensitizer. In SDT, the small treatment region by a single shot of HIFU results in a long treatment time, which needs to be reduced by improving the efficiency of ROS generation. ROS can be visualized by the sonochemiluminescence of luminol. In this study, a polyacrylamide gel soaked with luminol solution is used as a target of ultrasonic exposure. The effect of the duty cycle of HIFU exposure on ROS generation was investigated by capturing sonochemiluminescent light emission, and high-speed photography of cavitation bubbles.