Electrochemical Aspects of Electrospray and Laser Desorption/Ionization for Mass Spectrometry
Electrochemical Aspects of Electrospray and Laser Desorption/Ionization for Mass Spectrometry
复制标题
DOI：
10.1146/annurev.anchem.111808.073740
复制
发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Girault, Hubert H.
中科院分区：
文献类型：
--
作者：
Abonnenc, Melanie;Qiao, Liang;Girault, Hubert H.
软电离方法，即电喷雾电离和激光解吸/电离，被广泛用于将大分子作为完整的气相离子从溶液或固体基质中转移。在这两个过程中，都发生了源内电化学和光电化学反应。这些发生在界面上的电极反应对电离产物有重要的影响，但很少引起人们的注意。我们表明，对这两种类型的电化学反应有良好的控制可以导致新的分析应用。例子包括通过接枝质量标签的在线标记和多肽的源内光氧化。
Soft-ionization methods, namely electrospray ionization and laser desorption/ionization, are widely used to transfer large molecules as intact gas-phase ions either from a solution or from a solid substrate. During both processes, in-source electrochemical and photoelectrochemical reactions occur. These electrode reactions, which take place at interfaces, play important roles in influencing the ionization products, but they have received little attention. We show that having good control over both types of electrochemical reactions can lead to new analytical applications. Examples include online tagging by grafting of mass tags and in-source photooxidation of peptides.