VARIANT SUBLINES WITH DIFFERENT METASTATIC POTENTIALS SELECTED IN NUDE-MICE FROM HUMAN ORAL SQUAMOUS-CELL CARCINOMAS
VARIANT SUBLINES WITH DIFFERENT METASTATIC POTENTIALS SELECTED IN NUDE-MICE FROM HUMAN ORAL SQUAMOUS-CELL CARCINOMAS
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DOI：
10.1111/j.1600-0714.1989.tb01570.x
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发表时间：
1989-08-01
影响因子：
3.3
通讯作者：
SASAKI, S
中科院分区：
文献类型：
--
作者：
MOMOSE, F;ARAIDA, T;SASAKI, S
具有转移潜力的变体亚系LMF3、LMF4和LMF5是从人口腔鳞状癌细胞系HSC-3建立的。这些亚系在皮下接种裸鼠后转移至引流淋巴结。它们是通过体内连续选择从亲本HSC-3获得的。在每个步骤中，培养转移至淋巴结的细胞并重新接种到裸鼠中。另外两种细胞系 HSC-2 和 HSC-4 也是从其他患者身上建立的，它们既不具有侵袭性也不具有转移潜力。比较高转移、低转移和非转移细胞的生物学特性。转移细胞迅速生长并侵入接种部位的周围组织。在选定的变体中，静脉注射肿瘤细胞后肺部定植的发生率很高。转移细胞在 I 型胶原基质中形成弥漫性集落，并且更倾向于粘附 IV 型胶原网络。
Variant sublines LMF3, LMF4 and LMF5 and with metastatic potential were established from a human oral squamous carcinoma cell line HSC-3. These sublines metastasized to the draining lymph nodes after subcutaneous inoculation into nude mice. They were obtained by sequential selction in vivo from the parent HSC-3. At each step, the cells which metastasized to lymph nodes were cultured and reinoculated into nude mice. Two other cell lines HSC-2 and HSC-4 were also established from other patients, that had neither invasive nor metastatic potential. Biologic properties were compared among high metastatic, low metastatic and non-metastatic cells. Metastatic cells grew rapidly and invaded into surrounding tissues at the inoculated site. The incidence of pulmonary colonization after intravenous injection of tumor cells was high in selected variants. Metastatic cells formed diffuse colonies in type I collagen matrix and had a higher tendency to adhere to type IV collagen network.