Biomimetic autoseparation of leukocytes from whole blood in a microfluidic device
Biomimetic autoseparation of leukocytes from whole blood in a microfluidic device
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DOI：
10.1021/ac049037i
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发表时间：
2005-02-01
影响因子：
7.4
通讯作者：
Bitensky, MW
中科院分区：
文献类型：
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作者：
Shevkoplyas, SS;Yoshida, T;Bitensky, MW
白细胞占所有血细胞的不到1%。从全血样品开始富集它们的数量，是许多临床和基础研究测定所需的第一步。我们创造了一种微流体装置，该装置利用微循环中血流的固有特征，例如血浆撇除和白细胞边缘化，直接从全血中分离白细胞。它由一个简单的矩形微通道网络组成，旨在增强白细胞的侧向迁移及其随后从侧壁附近的红细胞耗尽区域的提取。单次通过该装置产生白细胞与红细胞比率的34倍富集。它对微升全血样本进行操作，提供白细胞的阳性、连续流动选择，并且既不需要细胞的初步标记，也不需要能量输入（除了支持血液流动的小压力梯度）。这种简单、高效且廉价的技术可用作初始全血样品制备的芯片实验室组件。将其整合到需要白细胞富集的微量分析设备中，将使这些设备加速过渡到现场进行即时临床检测。
Leukocytes comprise less than 1% of all blood cells. Enrichment of their number, starting from a sample of whole blood, is the required first step of many clinical and basic research assays. We created a microfluidic device that takes advantage of the intrinsic features of blood flow in the microcirculation, such as plasma skimming and leukocyte margination, to separate leukocytes directly from whole blood. It consists of a simple network of rectangular microchannels designed to enhance lateral migration of leukocytes and their subsequent extraction from the erythrocyte-depleted region near the sidewalls. A single pass through the device produces a 34-fold enrichment of the leukocyte-to-erythrocyte ratio. It operates on microliter samples of whole blood, provides positive, continuous flow selection of leukocytes, and requires neither preliminary labeling of cells nor input of energy (except for a small pressure gradient to support the flow of blood). This effortless, efficient, and inexpensive technology can be used as a lab-on-a-chip component for initial whole blood sample preparation. Its integration into microanalytical devices that require leukocyte enrichment will enable accelerated transition of these devices into the field for point-of-care clinical testing.