Quantification of ZAP70 mRNA in B cells by real-time PCR is a powerful prognostic factor in chronic lymphocytic leukemia
Quantification of ZAP70 mRNA in B cells by real-time PCR is a powerful prognostic factor in chronic lymphocytic leukemia
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DOI：
10.1373/clinchem.2007.089326
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发表时间：
2007-10-01
影响因子：
9.3
通讯作者：
Lagneaux, Laurence
中科院分区：
文献类型：
--
作者：
Stamatopoulos, Basile;Meuleman, Nathalie;Lagneaux, Laurence
背景：慢性淋巴细胞白血病（CLL）的预后和临床结果具有异质性。免疫球蛋白可变重链区 (IGHV) 的突变状态已被用来根据总生存 (CIS) 和临床特征将患者分为 2 组，但这种耗时分析的劳动密集型性质和成本促使人们对替代标记进行研究。方法：我们开发了一种标准化的定量实时逆转录 PCR (qPCR) 方法来测量纯化中的 zeta 链 (TCR) 相关蛋白激酶 (ZAP70) mRNA CD19(+)细胞。我们在 108 名患者（中位随访时间为 82 个月）的队列中评估了该方法和其他方法（ZAP70 和 CD38 蛋白的流式细胞术分析以及脂蛋白脂肪酶 mRNA 的 qPCR 分析），以评估与 IGHV 突变状态、OS 和无治疗生存 (TFS) 的任何关联。结果：qPCR 测量的 ZAP70 和 IGHV 突变状态之间的关联具有统计学显着性 [X-2 (1) = 50.95；磷
Background: Chronic lymphocytic leukemia (CLL) is heterogeneous with respect to prognosis and clinical outcome. The mutational status of the immunoglobulin variable heavy chain region (IGHV) has been used to classify patients into 2 groups in terms of overall survival (CIS) and clinical characteristics, but the labor-intensive nature and the cost of this time-consuming analysis has prompted investigations of surrogate markers.Methods: We developed a standardized quantitative real-time reverse transcription-PCR (qPCR) method to measure zeta-chain (TCR)-associated protein kinase (ZAP70) mRNA in purified CD19(+) cells. We evaluated this and other methods (flow cytometry analyses of ZAP70 and CD38 proteins and qPCR analysis of lipoprotein lipase mRNA) in a cohort of 108 patients (median follow-up, 82 months) to evaluate any associations with IGHV mutational status, OS, and treatment free survival (TFS).Results: The association between qPCR-measured ZAP70 and IGHV mutational status was statistically significant [X-2 (1) = 50.95; P