Additional evidence that pityriasis rosea is associated with reactivation of human herpesvirus-6 and-7
Additional evidence that pityriasis rosea is associated with reactivation of human herpesvirus-6 and-7
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DOI：
10.1111/j.0022-202x.2005.23719.x
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发表时间：
2005-06-01
影响因子：
6.5
通讯作者：
Malnati, MS
中科院分区：
文献类型：
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作者：
Broccolo, F;Drago, F;Malnati, MS
为了阐明人类疱疹病毒(HHV)-6和-7(HHV-7)在玫瑰糠疹(PR)中的作用，我们用实时定量聚合酶链式反应(RT-PCR)方法检测了它们在血浆、外周血单个核细胞(PBMC)和组织中的DNA载量。我们还用免疫组织化学的方法研究了皮肤中HHV-6和HHV-7的特异性抗原，并用合体抑制试验研究了抗HHV-7的中和活性。采集31例PR患者(14例儿童，17例成人)、12例其他皮肤病患者和36例献血员的血浆和PBMC。皮肤活检取自15例成人PR患者和12例其他皮炎患者。PR血浆中HHV-6和HHV-7DNA的阳性率分别为17%和39%，而对照组均为阴性。HHV-7病毒血症与较高的PBMC载量有关，在成年人中，与全身症状有关。PR患者PBMC中HHV-7水平高于对照组，但不高于对照组。HHV-6和HHV-7抗原仅在PR皮肤中发现(分别占分析患者的17%和67%)，表明存在生产性感染。PR患者和对照组均可检测到合胞体中和抗体，但HHV-7病毒血症患者的合胞体中和抗体滴度较低。这些数据证实了PR与活动性HHV-7或较小程度的HHV-6感染之间的因果关系。
To elucidate the role of human herpesvirus (HHV)-6 and -7 (HHV-7) in pityriasis rosea (PR), we measured their DNA load in plasma, peripheral blood mononuclear cells (PBMC), and tissues using a calibrated quantitative real-time PCR assay. We also studied HHV-6- and HHV-7-specific antigens in skin by immunohistochemistry and anti-HHV-7 neutralizing activity using a syncytia-inhibition test. Plasma and PBMC were obtained from 31 PR patients (14 children, 17 adults), 12 patients with other dermatites, and 36 blood donors. Skin biopsies were obtained from 15 adults with PR and 12 with other dermatites. HHV-6 and HHV-7 DNA were detected in 17% and in 39% of PR plasmas, respectively, but in no controls. HHV-7 viremia was associated with a higher PBMC load and, in adults, with systemic symptoms. HHV-7, but not HHV-6, levels in PBMC were higher in PR patients than in controls. HHV-6 and HHV-7 antigens were found only in PR skin (17% and 67% of patients analyzed, respectively), indicating a productive infection. Syncytia-neutralizing antibodies were found in PR patients and controls, but their titers were lower in patients with HHV-7 viremia. These data confirm the causal association between PR and active HHV-7 or, to a lesser extent, HHV-6 infection.