MEMBRANE-FUSION IN PROKARYOTES - BACTERIOPHAGE-PHI-6 MEMBRANE FUSES WITH THE PSEUDOMONAS-SYRINGAE OUTER-MEMBRANE
MEMBRANE-FUSION IN PROKARYOTES - BACTERIOPHAGE-PHI-6 MEMBRANE FUSES WITH THE PSEUDOMONAS-SYRINGAE OUTER-MEMBRANE
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DOI：
10.1002/j.1460-2075.1987.tb02388.x
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发表时间：
1987-05-01
期刊：
影响因子：
11.4
通讯作者：
SOMERHARJU, PJ
中科院分区：
文献类型：
--
作者：
BAMFORD, DH;ROMANTSCHUK, M;SOMERHARJU, PJ
用含脂包膜细菌病毒。vphi描述了原核生物系统中蛋白触发的膜融合。6和它的宿主，革兰氏阴性细菌丁香假单胞菌。噬菌体颗粒可以融合形成多个颗粒，其中两个或多个核衣壳被单个膜囊泡包围，其体积与融合颗粒的数量成正比。为了使融合发生，参与的噬菌体颗粒的膜中需要一种融合蛋白。感染宿主细胞后，病毒膜与细菌膜发生融合，而质周酶碱性磷酸酶不会渗漏到细胞外上清液中。在感染后5 - 20分钟，荧光噬菌体磷脂与细菌膜脂发生时间依赖性混合。噬菌体膜蛋白和磷脂与感染细胞的细菌外膜共同纯化。这种融合不受二价阳离子和pH值的影响，类似仙台病毒与质膜的融合。这是在原核生物中描述的第一个靶向的、依赖蛋白质的融合事件。
Protein-triggered membrane fusion in the prokaryotic system is described using the lipid-containing enveloped bacterial virus .vphi.6 and its host, the Gram-negative bacterium Pseudomonas syringae. Bacteriophage particles can be fused to form multiple particles where two or more nucleocapsids are surrounded by a single membrane vesicle with a volume proportional to the number of fused particles. For fusion to occur, a fusogenic protein is required in the membrane of the participating phage particles. Upon infection of the host cell, fusion of the viral membrane with the bacterial membrane takes place without leakage of the periplasmic enzyme alkaline phosphatase to the extracellular supernatant. There is a time-dependent mixing of fluorescent phage phospholipids with the bacterial membrane lipids between 5 and 20 min post-infection. The phage membrane proteins and phospholipids co-purify with the bacterial outer membrane of infected cells. The fusion is independent of divalent cations and pH, resembling Sendai virus fusion with the plasma membrane. This is the first targeted, protein-dependent fusion event described in prokaryotes.