Identification and molecular analysis of cashmere goat lncRNAs reveal their integrated regulatory network and potential roles in secondary hair follicle
Identification and molecular analysis of cashmere goat lncRNAs reveal their integrated regulatory network and potential roles in secondary hair follicle
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绒山羊 lncRNA 的鉴定和分子分析揭示了它们的整合调控网络及其在次级毛囊中的潜在作用
DOI：
10.1080/10495398.2020.1747477
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发表时间：
2020-03-31
影响因子：
3.7
通讯作者：
Bai, Wen L.
中科院分区：
文献类型：
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作者：
Jiao, Qian;Wang, Yan R.;Bai, Wen L.
长链非编码RNA（lncRNA）是一类长度超过200 bp的真核转录本。它们有助于调节涉及多个过程的基因表达，包括皮肤细胞增殖、分化和次级毛囊 (SHF) 的重建。在本研究中，我们首先根据 NCBI 数据库中的 EST 序列，从绒山羊 SHF 中鉴定出 16 个推定的 lncRNA。其次，我们研究了它们在绒山羊SHF中的转录模式，并构建了它们的ceRNA调控网络。 RT-qPCR结果显示，4个lncRNA（lncRNA-475074、-052149、-052140和-051789）显着上调，9个lncRNA（lncRNA-711032、-475083、-475070、-052139、-052127、-052037、 -051903、-051847 和 -051804) 在绒山羊的生长期 SHF 中显着下调。 CeRNA 网络分析揭示了 lncRNA、miRNA 和 mRNA 之间复杂的相互作用关系。进一步，基于生物信息学技术对鉴定出的lncRNA的潜在靶基因进行KEGG通路富集，结果表明差异表达的lncRNA可能直接或间接调控潜在靶基因。本研究结果将为进一步探索绒山羊SHF中已鉴定的lncRNA的功能和可能机制提供重要信息。
Long non-coding RNAs (lncRNAs) is a class of eukaryotic transcripts with length of more than 200 bp. They contribute to the regulation of gene expressions involved in multiple processes including the skin cell proliferation, differentiation, and reconstruction of the secondary hair follicles (SHFs). In this study, firstly, we identified 16 putative lncRNAs from SHFs of cashmere goat based on the EST sequences from NCBI database. Secondly, we investigated their transcriptional pattern in SHFs of cashmere goat, and constructed their ceRNA regulatory networks. The RT-qPCR results showed four lncRNAs (lncRNA-475074, -052149, -052140, and -051789) were significantly up-regulated, and nine lncRNAs (lncRNA-711032, -475083, -475070, -052139, -052127, -052037, -051903, -051847, and -051804) were significantly down-regulatd in anagen SHFs of cashmere goat. CeRNA networks analysis revealed complex interactional relationship among lncRNAs, miRNAs and mRNAs. Further, the KEGG pathway enrichment was performed for the potential target genes of the identified lncRNAs based on bioinformatics technique, and the results indicated that differentially expressed lncRNAs directly or indirectly might regulate potential target genes. Our results from this study will provide a significant information for further exploring the functions and possible mechanisms of the identified lncRNAs in SHFs of cashmere goat.