Original LncRNA MEG3 regulates breast cancer proliferation and apoptosis through miR-141-3p/RBMS3 axis
Original LncRNA MEG3 regulates breast cancer proliferation and apoptosis through miR-141-3p/RBMS3 axis
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DOI：
10.1016/j.ygeno.2021.04.015
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发表时间：
2021-04-21
期刊：
影响因子：
4.4
通讯作者：
Shi, Yonggang
中科院分区：
文献类型：
--
作者：
Dong, Shiliang;Ma, Minrui;Shi, Yonggang
母体表达3(MEG3)和RNA结合基序单链相互作用蛋白3(RBMS3)在乳腺癌易感基因(BRCA)中异常表达，但两者在乳腺癌中的作用机制尚不清楚。通过体内和体外实验，我们发现MEG3和RBMS3在BC中低表达，与miR-141-3p高表达呈负相关，相互之间呈正相关。MEG3靶向miR-141-3p，miR-141-3p靶向RBMS3。MEG3主要分布于BC细胞质，可下调miR-141-3p，上调RBMS3，逆转miR-141-3p对相关基因表达的影响，促进肿瘤的发生发展。过表达MEG3抑制移植瘤生长，通过调节凋亡相关因子促进细胞凋亡，上调RBMS3的表达，下调miR-141-3p的表达。结果表明，MEG3通过miR-141-3p/RBMS3轴抑制BC细胞增殖和促进细胞凋亡，MEG3通过miR-141-3p抑制移植瘤生长。
Maternally expressed 3 (MEG3) and RNA binding motif single stranded interacting protein 3 (RBMS3) are abnormally expressed in breast cancer susceptibility genes (BRCA), but the mechanism of the two in breast cancer (BC) is unclear. By performing in vivo and in vitro experiments, we found that MEG3 and RBMS3 were low-expressed, negatively correlated with high-expressed miR-141-3p, were positively correlated with each other in BC. MEG3 targeted miR-141-3p, and miR-141-3p targeted RBMS3. MEG3, which was mainly distributed in BC cytoplasm, could down-regulate miR-141-3p and up-regulate RBMS3, and reverse effect of miR-141-3p on related gene expressions and on promoting cancer development. Overexpressed MEG3 inhibited growth of xenografts, promoted cell apoptosis via regulating apoptosis related factors, and up-regulated RBMS3 expression but down-regulated miR-141-3p. The findings of this study showed that MEG3 inhibited proliferation and promoted apoptosis of BC cells through the miR-141-3p/RBMS3 axis, and MEG3 inhibited growth of xenografts through miR-141-3p.