Upregulated expression of human neutrophil peptides 1, 2 and 3 (HNP 1-3) in colon cancer serum and tumours: a biomarker study.
Upregulated expression of human neutrophil peptides 1, 2 and 3 (HNP 1-3) in colon cancer serum and tumours: a biomarker study.
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DOI：
10.1186/1471-2407-5-8
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发表时间：
2005-01-19
期刊：
影响因子：
3.8
通讯作者：
Raskov H
中科院分区：
文献类型：
--
作者：
Albrethsen J;Bøgebo R;Gammeltoft S;Olsen J;Winther B;Raskov H
需要用于局部结肠肿瘤和治疗后预后的分子标记。蛋白质组学研究目前正在产生大量具有临床潜力的生物标志物研究。我们研究血浆和肿瘤提取物的蛋白质组成，目的是鉴定结肠癌的生物标志物。通过表面增强激光解吸/电离 - 飞行时间/质谱 (SELDI-TOF/MS)，我们将结肠癌血清的蛋白质谱与健康个体的血清以及结肠肿瘤与正常结肠组织的蛋白质谱进行比较。通过尺寸排阻色谱法，我们研究了 HNP 1-3 与高质量血浆蛋白的结合。通过微流，我们研究了 HNP 1-3 对哺乳动物细胞的影响。人中性粒细胞肽 -1、-2 和 -3 (HNP 1-3)，也称为阿尔法防御素-1、-2 和 -3，在结肠癌患者的血清和结肠肿瘤的蛋白质提取物中浓度较高。血清中的一部分 HNP 1-3 与未鉴定的高质量血浆蛋白结合。从结肠肿瘤中纯化的 HNP 1-3 对哺乳动物细胞具有致命性。 HNP 1-3 可以与其他诊断工具结合作为结肠癌的血液标记物。我们建议 HNP 1-3 通过附着在肿瘤微环境中的高质量血浆蛋白而进入血流。我们讨论了 HNP 1-3 对肿瘤进展的影响。
Molecular markers for localized colon tumours and for prognosis following therapy are needed. Proteomics research is currently producing numerous biomarker studies with clinical potential. We investigate the protein composition of plasma and of tumour extracts with the aim of identifying biomarkers for colon cancer. By Surface Enhanced Laser Desorption/Ionisation – Time Of Flight / Mass spectrometry (SELDI-TOF/MS) we compare the protein profiles of colon cancer serum with serum from healthy individuals and the protein profiles of colon tumours with normal colon tissue. By size exclusion chromatography, we investigate the binding of HNP 1-3 to high mass plasma proteins. By microflow we investigate the effect of HNP 1-3 on mammalian cells. Human Neutrophil Peptides -1, -2 and -3 (HNP 1-3), also known as alfa-defensin-1, -2 and -3, are present in elevated concentrations in serum from colon cancer patients and in protein extracts from colon tumours. A fraction of HNP 1-3 in serum is bound to unidentified high mass plasma proteins. HNP 1-3 purified from colon tumours are lethal to mammalian cells. HNP 1-3 may serve as blood markers for colon cancer in combination with other diagnostic tools. We propose that HNP 1-3 are carried into the bloodstream by attaching to high mass plasma proteins in the tumour microenvironment. We discuss the effect of HNP 1-3 on tumour progression.