Methyl jasmonate induced defense responses increase resistance to Fusarium oxysporum f. sp cubense race 4 in banana
Methyl jasmonate induced defense responses increase resistance to Fusarium oxysporum f. sp cubense race 4 in banana
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DOI：
10.1016/j.scienta.2013.10.011
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发表时间：
2013-12-17
影响因子：
4.3
通讯作者：
Xie, Jianghui
中科院分区：
文献类型：
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作者：
Sun, Dequan;Lu, Xinhua;Xie, Jianghui
香蕉枯萎病（Fusarium wilt）是一种严重的香蕉（Musa spp.）根系病害，目前威胁着全世界香蕉的生产。茉莉酸甲酯（MeJA）因其诱导系统防御各种病原体的能力而得到认可。在本研究中，我们用1.5 mM外源MeJA处理香蕉植株。研究了不同处理对几丁质酶、β -1,3-葡聚糖酶、总酚（TP）、过氧化氢（H2O2）、超氧自由基（O-2(-)）、丙二醛（MDA）水平的影响，以及超氧化物歧化酶（SOD）、过氧化物酶（POD）、多酚氧化酶（PPO）、过氧化氢酶（CAT）和苯丙氨酸解氨酶（PAL）活性的影响，以及疾病发病率（DI）和疾病严重程度（DS）的影响。结果表明，MeJA能调节香蕉根系中H2O2和O-2（-）含量，降低MDA含量。同时，MeJA处理香蕉植株的几丁质酶、β -1,3-葡聚糖酶和TP含量显著提高，SOD、POD、PPO、CAT和PAL活性显著提高，且MeJA处理显著降低了香蕉植株的发病率（DI）和疾病严重程度（DS）。清除性抗氧化酶活性的提高导致细胞活性氧（ROS）水平的降低，而活性氧对维持细胞膜的完整性至关重要。处理根中致病相关蛋白、几丁质酶和β -1,3-葡聚糖酶的积累以及总酚和PAL活性也表明MeJA触发了次生代谢物生物合成途径的关键酶，表明MeJA参与了疾病相关防御系统的激活。(C) 2013 Elsevier B.V.版权所有
Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum, is a severe banana (Musa spp.) root disease, currently threatening banana production worldwide. Methyl jasmonate (MeJA) has been recognized for its ability to induce systemic defense against various pathogens. In this study, we treated banana plantlets with 1.5 mM exogenous MeJA. The effects of treatment on the level of chitinase, beta-1,3- glucanase, total phenols (TP), hydrogen peroxide (H2O2), superoxide radicals (O-2(-)), malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), catalase (CAT) and phenylalanine ammonia-lyase (PAL), plus disease incidence (DI) and disease severity (DS) were investigated. The results demonstrated that MeJA regulated the contents of H2O2 and O-2(-), and decreased MDA in banana roots. Meanwhile, plantlets treated with MeJA exhibited considerably higher chitinase, beta-1,3-glucanase and TP contents, and higher activities of SOD, POD, PPO, CAT and PAL In addition, MeJA treatment dramatically reduced disease incidence (DI) and disease severity (DS) of banana plants compared to control. The higher activities of scavenger antioxidant enzymes account for the reduction of cellular levels of reactive oxygen species (ROS), which is of vital importance for maintaining cell membrane integrity. The accumulation of pathogenesis-related proteins, chitinases and beta-1,3-glucanase, as well as total phenols and PAL activity in the treated roots also indicated that MeJA triggered key enzymes of secondary metabolite biosynthetic pathways, suggesting that MeJA was involved in the activation of a disease-related defense system. (C) 2013 Elsevier B.V. All rights reserved.