Microbiological Parameters in the Primary Production of Berries: A Pilot Study
Microbiological Parameters in the Primary Production of Berries: A Pilot Study
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DOI：
10.3390/foods7070105
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发表时间：
2018-07-01
期刊：
影响因子：
5.2
通讯作者：
Decastelli, Lucia
中科院分区：
文献类型：
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作者：
Macori, Guerrino;Gilardi, Giovanna;Decastelli, Lucia
新鲜软水果的初级生产被认为是导致2013-2014年欧洲甲型肝炎病毒（HAV）大爆发的冷冻浆果污染的可疑关键点。在这项研究中，意大利浆果的生产领域进行了研究，其农业技术特点，并分析了新鲜水果肠道病毒（甲型肝炎病毒和诺如病毒（NoV）基因组I和基因组II（GGI和GGII））的存在，卫生质量参数的计数，和细菌病原体的流行。共对50个生产者进行了抽样调查，他们专门从事浆果的独家或共享种植。在两个黑莓样品中检测到大肠杆菌，而没有检测到甲型肝炎病毒和诺如病毒。样本对沙门氏菌属呈阴性，单核细胞增生李斯特菌和产滋贺毒素大肠杆菌（STEC）。除了大肠杆菌的存在外，农场的属性与阳性样品无关。大肠杆菌和黑莓嗜温菌的相关性有统计学意义。在蓝莓中，高好氧嗜温菌计数可能与外层对处理的抗性有关。然而，这两种病原体（沙门氏菌属）和STEC）和目标病毒（HAV，NoV GGI和GGII）均未检出，这突出表明在本试点研究所考虑的区域内，浆果食物链初级生产阶段食源性病原体和病毒污染的风险较低。
The primary production of fresh soft fruits was considered to be a suspected critical point for the contamination of frozen berries that were responsible for the large 2013-2014 Hepatitis A virus (HAV) outbreak in Europe. In this study, an Italian berries' production area was studied for its agro-technical characteristics, and the fresh fruits were analyzed for the presence of enteric viruses (HAV and Norovirus (NoV) genogroup I and genogroup II (GGI and GGII)), the enumeration of hygienic quality parameters, and the prevalence of bacterial pathogens. A total of 50 producers were sampled, who specialized in the exclusive or shared cultivation of berries. Escherichia coli was detected in two blackberry samples, whereas HAV and Norovirus were not detected. The samples were negative for Salmonella spp., Listeria monocytogenes, and Shiga toxin-producing Escherichia coli (STEC). The farms' attributes were not associated with positive samples, apart from the presence of E. coli and the aerobic mesophilic bacteria for blackberry that were statistically correlated. In blueberries, the high aerobic mesophilic count could likely be associated with the resistance of the outer layer to handling. However, the two pathogens (Salmonella spp. and STEC) and the targeted viruses (HAV, NoV GGI and GGII) were not detected, highlighting the low risk of foodborne pathogens and viral contamination at the primary production stage of the berry food chain in the area considered in this pilot study.