IDENTIFICATION OF A DEVELOPMENTALLY-REGULATED TRANSCRIPT EXPRESSED DURING ENCYSTATION OF ENTAMOEBA-INVADENS
IDENTIFICATION OF A DEVELOPMENTALLY-REGULATED TRANSCRIPT EXPRESSED DURING ENCYSTATION OF ENTAMOEBA-INVADENS
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DOI：
10.1016/0166-6851(94)90102-3
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发表时间：
1994-09-01
影响因子：
1.5
通讯作者：
EICHINGER, D
中科院分区：
文献类型：
--
作者：
SANCHEZ, L;ENEA, V;EICHINGER, D
内阿米巴属的滋养体分化为包囊的过程在形态学上和生物化学上都有描述，但阶段特异性基因表达的研究还未见报道。侵袭内阿米巴是目前唯一可以在无菌培养中诱导分化的物种，是溶组织内阿米巴的一个有用的模型系统。使用这个模型系统，我们进行了cDNA-mRNA杂交实验，以比较从滋养体和寄生虫在不同阶段的包囊形成的RNA群体。我们检测到的积累人口的阶段特异性转录后8和22小时之间的寄生虫被放置在诱导培养基中。为了确定参与滋养体-包囊转化的基因，我们进行了差异筛选的cDNA文库。这产生了一个代表阶段特异性基因的克隆，其转录物在植物生长的滋养体和成熟的包囊中几乎检测不到，但在包囊形成开始时很容易检测到。描述了该基因的其他特征及其预测的蛋白质产物。
Differentation of trophozoites into cysts in Entamoeba species has been described morphologically and to a lesser extent biochemically, but studies of stage specific gene expression have not been reported. At present Entamoeba invadens is the only species that can be induced to differentiate in axenic culture and is a useful model system for the human parasite Entamoeba histolytica. Using this model system, we performed cDNA-mRNA hybridization experiments to compare the RNA populations from trophozoites and from parasites at different stages of cyst formation. We detected the accumulation of a population of stage specific transcripts between 8 and 22 h after parasites are placed in induction medium To identify genes involved in the trophozoite-cyst transformation we carried out a differential screening of a cDNA library. This yielded a clone that represents a stage specific gene whose transcripts are barely detectable in vegetatively grown trophozoites and maturing cysts, but are readily detected at the onset of cyst formation. Other features of the gene and its predicted protein product(s) are described.