Dual-emission fluorescent probe templated by spherical polyelectrolyte brush for ratiometric detection of copper ions
Dual-emission fluorescent probe templated by spherical polyelectrolyte brush for ratiometric detection of copper ions
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DOI：
10.1007/s10853-020-04757-6
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发表时间：
2020-05
影响因子：
4.5
通讯作者：
Zhishuang Ye;Li Li-Li;Fang Zhao;Qingsong Yang;Yunwei Wang;K. Bohinc;Xuhong Guo
中科院分区：
文献类型：
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作者：
Zhishuang Ye;Li Li-Li;Fang Zhao;Qingsong Yang;Yunwei Wang;K. Bohinc;Xuhong Guo
在球形聚电解质电刷（SPB）中制备了一种新型的Cu2+比例荧光探针，将红光发射的铕配合物Eu(TTA)3Phen嵌入聚苯乙烯（PS）芯中产生参考信号，将绿色发射的谷胱甘肽覆盖的CdTe量子点固定在聚苯乙烯磺酸盐电刷壳上提供感应信号。结果表明，该探针对Cu2+具有较高的灵敏度和选择性。Cu2+的加入使探针在550 nm处的荧光猝灭，而在614 nm处的荧光信号保持不变。在优化条件下，两种荧光发射强度比在0 ~ 1000 nM范围内呈线性响应，Cu2+的检出限为1.45 nM。该探针具有响应快、成本低、稳定性好等优点，非常适用于铜污染的检测。SPB由于其特殊的核壳结构和富集反离子的能力，被证明是构建高质量比率探针的优良“载体”。这项工作为高质量比例荧光探针的开发提供了一种通用的方法，可以扩展到各个领域的潜在应用，如环境监测、生物成像、病理分析和癌症诊断。
A novel ratiometric fluorescent probe for Cu2+was fabricated in spherical polyelectrolyte brush (SPB) where the red-emission europium complex Eu(TTA)3Phen was embedded in the polystyrene (PS) core to generate the reference signal and the green-emission glutathione-capped CdTe quantum dot was immobilized onto the poly(styrene sulfonate) brush shell to provide the sensing signal. Results indicate that this probe has high sensitivity and selectivity for Cu2+over other metal ions. The addition of Cu2+could greatly quench the probe’s fluorescence at 550 nm, while the fluorescence signal at 614 nm kept unchanged. Under the optimized conditions, the intensity ratio of the two fluorescence emissions showed a linear response range from 0 to 1000 nM with a limit of detection of 1.45 nM for Cu2+. The probe is very suitable for the detection of copper contamination with quick response, low cost and great stability. SPB was proved to be an excellent “carrier” for building high-quality ratiometric probes due to its special core–shell structure and outstanding ability to enrich counterions. This work provides a versatile method for the development of high-quality ratiometric fluorescent probes, which can be extended to potential applications in various fields, such as environmental monitoring, biological imaging, pathological analysis and cancer diagnosis.