Analytical method for pyrethroid metabolites in urine of the non-occupationally exposed population by gas chromatography/mass spectrometry
Analytical method for pyrethroid metabolites in urine of the non-occupationally exposed population by gas chromatography/mass spectrometry
复制标题
非职业接触人群尿液中拟除虫菊酯代谢物气相色谱/质谱分析方法
DOI：
10.1093/chromsci/bmx048
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发表时间：
2017
影响因子：
1.3
通讯作者：
Toshiaki Yoshida
中科院分区：
文献类型：
--
作者：
阿部仁一郎;寺本 勲;Toshiaki Yoshida
拟除虫菊酯广泛用作家用杀虫剂或防蛀剂。描述了一种分析方法，用于测定尿液代谢物作为监测拟除虫菊酯暴露的生物标志物。总共11种尿代谢物，3-(2-羧基丙-1-烯基)-2,2-二甲基环丙烷甲酸、3-(2-氯-3,3,3-三氟丙-1-烯基)-2,2-二甲基环丙烷甲酸、3-(2,2-二氯乙烯基)-2,2-二甲基环丙烷甲酸、 2,2-二甲基-3-(2-甲基丙-1-烯基)环丙烷甲酸、4-氟-3-苯氧基苯甲酸、4-甲氧基甲基-2,3,5,6-四氟苯甲醇、2-甲基-3-苯基苯甲酸、4-甲基-2,3,5,6-四氟苯甲醇、3-苯氧基苯甲酸、2,3,5,6-四氟苯甲酸酸和2,2,3,3-四甲基环丙烷甲酸，进行酶水解并用甲苯萃取。转化为叔丁基二甲基甲硅烷基或三甲基甲硅烷基衍生物后，通过气相色谱/质谱法以电子轰击电离模式对它们进行分析。代谢物的校准曲线在尿液中 0–30 μg/L 的浓度范围内呈线性。可以准确、精确地测定它们（检测限：0.01-0.12 μg/L，定量限：0.04-0.41 μg/L）。收集的尿液样本可在-20°C冰箱中保存长达1个月。所提出的方法用于测定几名健康志愿者的尿液样本。该方法被认为可用于监测一般人群中拟除虫菊酯的暴露情况。
Pyrethroids are widely being used as household insecticides or mothproof repellents. An analytical method is described for determination of urinary metabolites as biomarkers for monitoring exposure to the pyrethroids. In total, 11 urinary metabolites, 3-(2-carboxyprop-1-enyl)-2,2-dimethylcyclopropanecarboxylic acid, 3-(2-chloro-3,3,3-trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylic acid, 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid, 2,2-dimethyl-3-(2-methylprop-1-enyl)cyclopropanecarboxylic acid, 4-fluoro-3-phenoxybenzoic acid, 4-methoxymethyl-2,3,5,6-tetrafluorobenzly alcohol, 2-methyl-3-phenylbenzoic acid, 4-methyl-2,3,5,6-tetrafluorobenzyl alcohol, 3-phenoxybenzoic acid, 2,3,5,6-tetrafluorobenzoic acid and 2,2,3,3-tetramethylcyclopropanecarboxylic acid, were enzymatically hydrolyzed and extracted with toluene. After transformation to theirtert-butyldimethylsilyl or trimethylsilyl derivatives, they were analyzed by gas chromatography/mass spectrometry in electron impact ionization mode. The calibration curves for the metabolite were linear over the concentration range of 0–30 μg/L in urine. They could be determined accurately and precisely (detection limits: 0.01–0.12 μg/L, quantification limits: 0.04–0.41 μg/L). The urine samples collected could be stored for up to 1 month at −20°C in a freezer. The proposed method was applied to determine urine samples from several health volunteers. The method was considered to be available for monitoring pyrethroid exposure in the general population.