Enzyme-targeted fluorescent small-molecule probes for bacterial imaging.
Enzyme-targeted fluorescent small-molecule probes for bacterial imaging.
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DOI：
10.1016/j.cbpa.2020.05.012
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发表时间：
2020-08
影响因子：
7.8
通讯作者：
Andrew P. Marshall;Joshua D. Shirley;Erin E. Carlson
中科院分区：
文献类型：
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作者：
Andrew P. Marshall;Joshua D. Shirley;Erin E. Carlson
传统上，用于可视化细菌中无数生化过程的分子成像方法依赖于结合荧光生物分子（例如融合蛋白）的分子生物学技术。这些方法有助于我们了解细菌的功能，但也有缺点，包括对天然蛋白质表达和功能的潜在干扰。为了克服这些限制，荧光小分子探针的使用受到了广泛的关注。在这里，我们从最近的文献中重点介绍了小分子探针在细菌细胞荧光成像中的应用，包括亲电探针、代谢探针和酶激活探针。尽管这些类型的化合物用于细菌成像仍然是相对较新的，但所选的例子表明，这些关键工具在探索细菌生理学方面具有令人兴奋的潜力。
Molecular imaging methods to visualize myriad biochemical processes in bacteria have traditionally been dependent upon molecular biology techniques to incorporate fluorescent biomolecules (e.g., fusion proteins). Such methods have been instrumental in our understanding of how bacteria function but are not without drawbacks, including potential perturbation to native protein expression and function. To overcome these limitations, the use of fluorescent small-molecule probes has gained much attention. Here, we highlight examples from the recent literature that showcase the utility of small-molecule probes for the fluorescence imaging of bacterial cells, including electrophilic, metabolic, and enzyme-activated probes. Although the use of these types of compounds for bacterial imaging is still relatively new, the selected examples demonstrate the exciting potential of these critical tools in the exploration of bacterial physiology.