Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton
Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton
复制标题
DOI：
10.1128/aem.68.6.2965-2971.2002
复制
发表时间：
2002-06
影响因子：
4.4
通讯作者：
K. Vrede;M. Heldal;S. Norland;G. Bratbak
中科院分区：
文献类型：
--
作者：
K. Vrede;M. Heldal;S. Norland;G. Bratbak
摘要海洋浮游细菌被分离并在分批培养中生长，直到它们的生长受到有机碳（C）、氮（N）或磷（P）的限制。在指数期和稳定期均从培养物中取样。用电子显微镜通过X射线微量分析分析单个细菌细胞的元素组成。还测量了细胞大小。元素含量在指数生长的细胞中最高（C细胞-1的149 ± 8 fg，N细胞-1的35 ± 2 fg，P细胞-1的12 ± 1 fg;所有分离株的平均值±标准误差）。在C限制细胞中发现最低的C含量（39 ± 3 fg的C细胞-1），在C和P限制细胞中发现最低的N含量（分别为12 ± 1和12 ± 2 fg的N细胞-1），在P限制细胞中发现最低的P含量（2.3 ± 0.6 fg的P细胞-1）。处理间的原子C：N比在3.8 ± 0.1和9.5 ± 1.0（平均值±标准误差）之间变化，C：P比在35 ± 2和178 ± 28之间变化，N：P比在6.7 ± 0.3和18 ± 3之间变化。由于不同类型的营养限制，碳体积比在菌株之间表现出很大的差异（从51± 4到241 ± 38 fg的C μm−1;单个菌株和处理的平均值±标准误差）。结果表明，不同的生长条件和细菌群落的差异可以解释以前报道的元素和碳体积比的变化。
ABSTRACT Marine bacterioplankton were isolated and grown in batch cultures until their growth became limited by organic carbon (C), nitrogen (N), or phosphorus (P). Samples were taken from the cultures at both the exponential and stationary phases. The elemental composition of individual bacterial cells was analyzed by X-ray microanalysis with an electron microscope. The cell size was also measured. The elemental content was highest in exponentially growing cells (149 ± 8 fg of C cell−1, 35 ± 2 fg of N cell−1, and 12 ± 1 fg of P cell−1; average of all isolates ± standard error). The lowest C content was found in C-limited cells (39 ± 3 fg of C cell−1), the lowest N content in C- and P-limited cells (12 ± 1 and 12 ± 2 fg of N cell−1, respectively), and the lowest P content in P-limited cells (2.3 ± 0.6 fg of P cell−1). The atomic C:N ratios varied among treatments between 3.8 ± 0.1 and 9.5 ± 1.0 (average ± standard error), the C:P ratios between 35 ± 2 and 178 ± 28, and the N:P ratios between 6.7 ± 0.3 and 18 ± 3. The carbon-volume ratios showed large variation among isolates due to different types of nutrient limitation (from 51± 4 to 241 ± 38 fg of C μm−1; average of individual isolates and treatments ± standard error). The results show that different growth conditions and differences in the bacterial community may explain some of the variability of previously reported elemental and carbon-volume ratios.