13-cis retinoyl-beta-glucuronide in lacrimal gland fluid of rabbits treated with 13-cis retinoic acid.
13-cis retinoyl-beta-glucuronide in lacrimal gland fluid of rabbits treated with 13-cis retinoic acid.
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用 13-顺式视黄酸处理的兔子泪腺液中的 13-顺式视黄酰-β-葡萄糖苷酸。
DOI：
10.1089/jop.1990.6.321
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Osgood,TB
中科院分区：
文献类型：
--
作者：
Ubels,JL;Osgood,TB
当兔子和人类口服 13-顺式视黄酸治疗时，视黄酸和极性更强的类视黄酸代谢物出现在眼泪和泪腺液中。这项研究检验了该代谢物是视黄酰-β-葡萄糖苷酸的假设。对用药理剂量的 13-顺式视黄酸治疗的兔子的泪腺液进行了分析。根据色谱保留时间、最大吸光度以及β-葡萄糖醛酸酶降解为13-顺式视黄酸，表明该类视黄醇代谢物是13-顺式视黄酰-β-葡萄糖醛酸。 13-顺式视黄酰-β-葡萄糖苷酸也从泪腺本身提取。结论是13-顺式视黄酰-β-葡萄糖醛酸是兔体内13-顺式视黄酸的主要代谢产物，并且这种类维生素A由泪腺分泌。
When rabbits and humans are treated with orally administered 13-cis retinoic acid, the retinoic acid and a more polar retinoid metabolite appear in tears and lacrimal gland fluid. This study tested the hypothesis that this metabolite was a retinoyl-β-glucuronide. Lacrimal gland fluid from rabbits treated with a pharmacologic dose of 13-cis retinoic acid was analyzed. Based on Chromatographic retention time, absorbance maximum, and degradation to 13-cis retinoic acid by the enzyme β-glucuronidase, it was shown that this retinoid metabolite is 13-cis retinoyl-β-glucuronide. The 13-cis retinoyl-β-glucuronide was also extracted from the lacrimal gland itself. It is concluded that 13-cis retinoyl-β-glucuronide is a major metabolite of 13-cis retinoic acid in the rabbit and that this retinoid is secreted by the lacrimal gland.