RNA polymerase II transcription factor SIII. I. Identification, purification, and properties.
RNA polymerase II transcription factor SIII. I. Identification, purification, and properties.
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DOI：
10.1016/s0021-9258(19)74431-7
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发表时间：
1993-12
期刊：
影响因子：
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通讯作者：
J. Bradsher;K. Jackson;R. Conaway;J. Conaway
中科院分区：
文献类型：
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作者：
J. Bradsher;K. Jackson;R. Conaway;J. Conaway
从大鼠肝细胞核提取物中鉴定并纯化了一种转录因子，该因子可刺激RNA聚合酶II准确启动转录本的合成。我们将该因子命名为SIII，其天然分子量约为140 kDa，由110、18和15 kDa的三种多肽组成。所有三种多肽都是重建SIII转录活性所必需的。SIII似乎不同于先前鉴定的哺乳动物转录因子。
A transcription factor that stimulates synthesis of accurately initiated transcripts by RNA polymerase II has been identified and purified to apparent homogeneity from rat liver nuclear extracts. This factor, which we designate SIII, has a native molecular mass of approximately 140 kDa and is composed of three polypeptides of 110, 18, and 15 kDa. All three polypeptides are required to reconstitute SIII transcriptional activity. SIII appears distinct from previously identified mammalian transcription factors.