TRIM25 inhibits spring viraemia of carp virus replication by positively regulating RIG-I signaling pathway in common carp (Cyprinus carpio L.)
TRIM25 inhibits spring viraemia of carp virus replication by positively regulating RIG-I signaling pathway in common carp (Cyprinus carpio L.)
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DOI：
10.1016/j.fsi.2022.06.033
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发表时间：
2022-06-27
影响因子：
4.7
通讯作者：
Shan，Shijuan
中科院分区：
文献类型：
--
作者：
Liu，Rongrong;Li，Hua;Shan，Shijuan
鲤鱼（Cyprinus carpioL.）是中国最广泛养殖的鱼类之一。鲤鱼春季病毒血症病毒（SVCV）是一种高致病性病毒，常造成池塘鲤鱼养殖的严重损失。天然免疫在抗病毒感染中起重要作用。为了更好地理解鲤鱼对SVCV感染的免疫应答，使用Illumina Novaseq 6000平台进行转录组分析。结果表明，SVCV感染后，RLR信号通路中的差异表达基因共3953条，表达量显著增加。随后，研究了RLR信号通路在SVCV感染中的作用。结果表明，鲤鱼RIG-I（CcRIG-I）和TRIM 25（CcTRIM 25）通过诱导TBK 1、IRF 3和p65的磷酸化以及fifn-1、viperin、isg 15和mx的表达，显著抑制SVCV的复制。进一步的研究表明，CcTRIM 25能够正向调节CcRIG-I介导的下游信号通路。最后，研究了CcTRIM 25促进CcRIG-I介导的信号转导的机制，CcTRIM 25可以与CcRIG-I的caspase激活和募集结构域（CARD）相互作用，促进CcRIG-I的K63连接的多聚泛素化。总之，本研究揭示了CcTRIM 25调控CcRIG-I介导的SVCV感染免疫应答的机制。
Common carp (Cyprinus carpioL.) is one of the most widely cultivated fish in China. Spring viraemia of carp virus (SVCV) is a highly pathogenic virus and has often caused excessive losses in carp pond fisheries. Innate immune play important roles against virus infection. To better understand the immune response of common carp against SVCV infection, transcriptome analysis was performed using the Illumina Novaseq 6000 platform. It was showed that a total of 3953 differentially expressed unigenes were identified, and the RLR signaling pathway were significantly enriched after SVCV infection. Subsequently, the role of RLR signaling pathway in SVCV infection was studied. The results showed that common carp RIG-I (CcRIG-I) and TRIM25 (CcTRIM25) significantly decreased the replication of SVCV by inducing the phosphorylation of TBK1, IRF3 and p65 and the expression ofifn-1,viperin,isg15andmx. Further studies illustrated thatCcTRIM25 could positive regulateCcRIG-I mediated downstream signaling pathway. Finally, the mechanism ofCcTRIM25 promotingCcRIG-I-mediated signaling was investigated.CcTRIM25 could interact with the caspase activation and recruitment domain (CARD) ofCcRIG-I and promoted K63-linked polyubiquitination ofCcRIG-I. Altogether, the study revealed a mechanism ofCcTRIM25 regulatingCcRIG-I mediated immune response in SVCV infection.