Concentration of Potassium in Plasma, Erythrocytes, and Muscle Tissue in Cows with Decreased Feed Intake and Gastrointestinal Ileus
Concentration of Potassium in Plasma, Erythrocytes, and Muscle Tissue in Cows with Decreased Feed Intake and Gastrointestinal Ileus
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采食量减少和胃肠道肠梗阻的奶牛血浆、红细胞和肌肉组织中钾的浓度
DOI：
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发表时间：
2016
影响因子：
2.6
通讯作者：
T. Wittek
中科院分区：
文献类型：
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作者：
S. Schneider;A. Müller;T. Wittek
健康奶牛消耗大量钾，食欲突然下降可导致低钾血症。评估钾稳态的常规方法是测量血浆或血清中的细胞外钾，但这并不能提供细胞内钾池的信息。假设/目的通过比较采食量减少和胃肠道肠梗阻奶牛细胞外和细胞内钾浓度来评估钾稳态。产后1 ~ 3天的奶牛20头（第一组）和胃肠道肠梗阻奶牛20头（第二组）。方法观察性横断面研究。用离子敏感电极测定血浆钾。用电感耦合等离子体发射光谱法测定红细胞和肌肉组织（肌肉活检）的细胞内钾。结果1组奶牛无低钾血症。胃肠道肠梗阻奶牛总体呈低钾血症（平均值±SD， 2.9 mmol/L±0.78），但红细胞和肌肉组织钾浓度不低于产后奶牛。红细胞胞内钾变化很大；第1组：3497-10735 mg/kg(5559±2002 mg/kg)，第2组：4139-21678 mg/kg（7473±4034 mg/kg）。1组（3356±735 mg/kg湿重）和2组（3407±1069 mg/kg湿重）肌肉组织钾含量无显著差异。细胞外和细胞内钾浓度之间没有关联。结论及临床意义血浆钾浓度测定不足以评价奶牛的钾代谢。
Background Healthy cows consume large amounts of potassium and a sudden loss in appetite can lead to hypokalemia. The routine method to evaluate potassium homeostasis is the measurement of the extracellular potassium in plasma or serum, but this does not provide information about the intracellular potassium pool. Hypothesis/Objectives To evaluate potassium homeostasis by comparing the extracellular and intracellular potassium concentration in cows with reduced feed intake and gastrointestinal ileus. Animals Twenty cows 1–3 days postpartum (group 1) and 20 cows with gastrointestinal ileus (group 2). Methods Observational cross‐sectional study. Plasma potassium was measured by using an ion‐sensitive electrode. Intracellular potassium was measured in erythrocytes and muscle tissue (muscle biopsy) by using inductively coupled plasma optical emission spectroscopy. Results Cows of group 1 did not have hypokalemia. Overall cows with gastrointestinal ileus were hypokalemic (mean ± SD, 2.9 mmol/L ± 0.78), but potassium concentration in erythrocytes and muscle tissue was not lower than in postpartum cows. Intracellular potassium in erythrocytes varied very widely; group 1: 3497–10735 mg/kg (5559 ± 2002 mg/kg), group 2: 4139–21678 mg/kg (7473 ± 4034 mg/kg). Potassium in muscle tissue did not differ between group 1 (3356 ± 735 mg/kg wet weight) and group 2 (3407 ± 1069 mg/kg wet weight). No association between extracellular and intracellular potassium concentrations was detected. Conclusions and clinical importance That measurement of plasma potassium concentration is not sufficient to evaluate potassium metabolism of cows.