Regulation of acrosome formation in mice expressing green fluorescent protein as a marker
Regulation of acrosome formation in mice expressing green fluorescent protein as a marker
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DOI：
10.1016/s0040-8166(00)80006-3
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发表时间：
2000-12-01
期刊：
影响因子：
2.6
通讯作者：
Parvinen, M
中科院分区：
文献类型：
--
作者：
Ventel채, S;Mulari, M;Parvinen, M
用顶体蛋白启动子下表达增强型绿色荧光蛋白的转基因小鼠研究了高尔基复合体和细胞骨架在体外生精上皮细胞周期的确切定义阶段的顶体系统早期发育过程中的作用。首先发现顶体蛋白在IV期粗线期精母细胞的细胞质中均匀表达，稳态水平增加到X期粗线期精母细胞，终动期初级精母细胞中，顶体酶开始聚集到高尔基复合体中。在精子发生的第2阶段，高尔基复合体的不同部位出现了几个小的荧光前顶体颗粒，并在第3阶段融合成一个实心的顶体系统。在培养的I-III期曲细精管节段中，诺考达唑减慢了顶体酶的掺入，并增加了顶体系统与细胞核的距离。促卵泡激素通过增加顶体系统的密度以及激活周围的微管网络而产生相反的作用。这些观察结果表明，微管在顶体系统的早期分化过程中具有重要的功能。
Transgenic mice expressing enhanced green fluorescent protein under acrosin promoter were used to study the role of the Golgi complex and of the cytoskeleton during early development of the acrosomic system in exactly defined stages of the seminiferous epithelial cycle during in vitro differentiation.First acrosin expression was found uniformly in the cytoplasm of stage IV pachytene spermatocytes, The steady-state level increased up to stage X pachytene spermatocytes, and in diakinetic primary spermatocytes, acrosin started to accumulate into the Golgi complex. During step 2 of spermiogenesis, several small fluorescent proacrosomic granules were seen in various parts of the Golgi complex, and they fused to a solid acrosomic system at step 3.In cultured stage I-III seminiferous tubule segments, nocodazole slowed down acrosin incorporation and increased the distance of the acrosomic system from the nucleus. Follicle stimulating hormone had an opposite effect by increasing density of the acrosomic system together with activation of the surrounding microtubule network. The observations suggest that microtubules have an important function during the early differentiation of the acrosomic system.