Severe streptomycin ototoxicity in the mouse utricle leads to a flat epithelium but the peripheral neural degeneration is delayed.
Severe streptomycin ototoxicity in the mouse utricle leads to a flat epithelium but the peripheral neural degeneration is delayed.
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链霉素对小鼠椭圆囊的严重耳毒性导致上皮扁平，但周围神经变性被延迟
DOI：
10.1016/j.heares.2017.09.004
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发表时间：
2017-11
期刊：
影响因子：
2.8
通讯作者：
Raphael Y
中科院分区：
文献类型：
--
作者：
Wang GP;Basu I;Beyer LA;Wong HT;Swiderski DL;Gong SS;Raphael Y
哺乳动物受损的前庭感觉上皮具有有限的自发毛细胞再生能力，这在很大程度上取决于存活的支持细胞的转分化。关于前庭支持细胞对严重损伤的反应知之甚少。在本研究中，我们评估了严重的耳毒性损伤对椭圆囊支持细胞的组织学的影响和神经支配的变化。我们将高剂量的链霉素注入小鼠后半规管，以诱导椭圆囊的严重损伤。塑料切片的扫描电子显微镜和光学显微镜均显示，在大多数样品中，上皮层的正常细胞结构被平坦的细胞层取代。免疫荧光染色显示，在严重受损的上皮层中有大量毛细胞和支持细胞标记物呈阴性的细胞。扁平上皮下的神经纤维在1个月时间点具有高密度，但到3个月时密度非常低。同样，前庭神经节神经元的数量在损伤后1个月没有变化，但在3个月时显著降低。因此，我们确定，小鼠椭圆囊上皮变成一个平坦的上皮细胞后，严重的病变，但神经成分的退化是缓慢的，这表明，治疗恢复平衡的毛细胞再生，干细胞治疗或前庭假体植入可能会受益于短期保存的神经基板。
The damaged vestibular sensory epithelium of mammals has a limited capacity for spontaneous hair cell regeneration, which largely depends on the transdifferentiation of surviving supporting cells. Little is known about the response of vestibular supporting cells to a severe insult. In the present study, we evaluated the impact of a severe ototoxic insult on the histology of utricular supporting cells and the changes in innervation that ensued. We infused a high dose of streptomycin into the mouse posterior semicircular canal to induce a severe lesion in the utricle. Both scanning electron microscopy and light microscopy of plastic sections showed replacement of the normal cytoarchitecture of the epithelial layer with a flat layer of cells in most of the samples. Immunofluorescence staining showed numerous cells in the severely damaged epithelial layer that were negative for hair cell and supporting cell markers. Nerve fibers under the flat epithelium had high density at the 1 month time point but very low density by 3 months. Similarly, the number of vestibular ganglion neurons was unchanged at 1 month after the lesion, but was significantly lower at 3 months. We therefore determined that the mouse utricular epithelium turns into a flat epithelium after a severe lesion, but the degeneration of neural components is slow, suggesting that treatments to restore balance by hair cell regeneration, stem cell therapy or vestibular prosthesis implantation will likely benefit from the short term preservation of the neural substrate.
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影响因子：
16.2
作者：
Mizutari K;Fujioka M;Hosoya M;Bramhall N;Okano HJ;Okano H;Edge AS
通讯作者：
Edge AS
影响因子：
2.8
作者：
Kawamoto K;Izumikawa M;Beyer LA;Atkin GM;Raphael Y
通讯作者：
Raphael Y
DOI：
10.1523/jneurosci.6274-11.2012
发表时间：
2012-05-09
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Burns JC;Cox BC;Thiede BR;Zuo J;Corwin JT
通讯作者：
Corwin JT
DOI：
10.1523/jneurosci.2057-11.2011
发表时间：
2011-10-26
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lin V;Golub JS;Nguyen TB;Hume CR;Oesterle EC;Stone JS
通讯作者：
Stone JS
DOI：
10.1083/jcb.137.6.1287
发表时间：
1997-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hasson T;Gillespie PG;Garcia JA;MacDonald RB;Zhao Y;Yee AG;Mooseker MS;Corey DP
通讯作者：
Corey DP