Exercise-induced enhancement of insulin sensitivity is associated with accumulation of M2-polarized macrophages in mouse skeletal muscle
Exercise-induced enhancement of insulin sensitivity is associated with accumulation of M2-polarized macrophages in mouse skeletal muscle
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DOI：
10.1016/j.bbrc.2013.10.005
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发表时间：
2013-11-08
影响因子：
3.1
通讯作者：
Watada, Hirotaka
中科院分区：
文献类型：
--
作者：
Ikeda, Shin-ichi;Tamura, Yoshifumi;Watada, Hirotaka
运动增强骨骼肌的胰岛素敏感性，但其机制尚不清楚。最近的数据表明，交替激活的M2巨噬细胞增强胰岛素靶器官如脂肪组织和肝脏中的胰岛素敏感性。因此，本研究的目的是确定抗炎M2巨噬细胞在运动诱导的骨骼肌胰岛素敏感性增强中的作用。C57 BL 6 J小鼠经历单次跑步机跑步（20 m/min，90 min）。24小时后，离体胰岛素刺激的2-脱氧葡萄糖摄取被发现在跖肌中增加。该变化与骨骼肌中表达CD 163的巨噬细胞（即M2极化巨噬细胞）数量增加相关。通过用含氯膦酸盐的脂质体预处理小鼠全身性消耗巨噬细胞，消除了骨骼肌中CD 163阳性巨噬细胞的积累以及运动后胰岛素敏感性的增强，而不影响胰岛素诱导的Akt和AS 160磷酸化或运动诱导的GLUT 4表达。这些结果表明，M2极化的巨噬细胞的积累参与运动诱导的小鼠骨骼肌胰岛素敏感性的增强，独立于Akt和AS 160的磷酸化和GLUT 4的表达。(C)2013 Elsevier Inc. All rights reserved.
Exercise enhances insulin sensitivity in skeletal muscle, but the underlying mechanism remains obscure. Recent data suggest that alternatively activated M2 macrophages enhance insulin sensitivity in insulin target organs such as adipose tissue and liver. Therefore, the aim of this study was to determine the role of anti-inflammatory M2 macrophages in exercise-induced enhancement of insulin sensitivity in skeletal muscle. C57BL6J mice underwent a single bout of treadmill running (20 m/min, 90 min). Twenty-four hours later, ex vivo insulin-stimulated 2-deoxy glucose uptake was found to be increased in plantaris muscle. This change was associated with increased number of CD163-expressing macrophages (i.e. M2-polarized macrophages) in skeletal muscle. Systemic depletion of macrophages by pretreatment of mice with clodronate-containing liposome abrogated both CD163-positive macrophage accumulation in skeletal muscle as well as the enhancement of insulin sensitivity after exercise, without affecting insulin-induced phosphorylation of Akt and AS160 or exercise-induced GLUT4 expression. These results suggest that accumulation of M2-polarized macrophages is involved in exercise-induced enhancement of insulin sensitivity in mouse skeletal muscle, independently of the phosphorylation of Akt and AS160 and expression of GLUT4. (C) 2013 Elsevier Inc. All rights reserved.