Plasma biomarkers in fish provide evidence for endocrine modulation in the Elbe River, Germany
Plasma biomarkers in fish provide evidence for endocrine modulation in the Elbe River, Germany
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DOI：
10.1021/es010186h
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发表时间：
2002-06-01
影响因子：
11.4
通讯作者：
Karbe, L
中科院分区：
文献类型：
--
作者：
Hecker, M;Tyler, CR;Karbe, L
采集野生团头鲂（Abramis brama L.）在德国的易北河，并分析了卵黄蛋白前体卵黄蛋白原（VTG），雌激素暴露的生物标志物，以及性类固醇11-酮睾酮（11 KT），睾酮（T）和17 β-雌二醇（E2），以研究内分泌调节的证据。此外，性腺状况和产卵结节的突出进行了调查。九个河流的网站进行了调查，易北河的影响，不同来源的内分泌活性物质。从一个没有家庭或工业排放的湖泊中收集真鲷作为对照。血浆VTG浓度显着较高的雄性鲷从捷克边境到易北河中部，在马格德堡附近和下游的德累斯顿（20和100倍之间高于控制），区域的特点是高水平的污水排放到河流中的位置采样的鱼的浓度最高。易北河后，从这个网站到大海，在男性血浆VTG浓度低于迈森，但仍高于对照组。11 KT和E2滴度在某些地点（接受最大工业排放的地点）的正常浓度下显示出异常。对性腺生长、成熟和血浆性类固醇的抑制作用与暴露于污染物（如有机锡、农药或金属）之间存在相互关系。然而，没有一种化学物质可以单独解释观察到的对性发育的抑制作用。结果表明，在易北河的延伸野生鲷的内分泌系统被打乱。
Blood plasma samples were collected from wild bream (Abramis brama L.) in the Elbe River, Germany, and analyzed for the yolk protein precursor vitellogenin (VTG), a biomarker for estrogen exposure, and the sex steroids 11-ketotestosterone (11KT), testosterone (T), and 17beta-estradiol (E2) to investigate for evidence of endocrine modulation. In addition,the gonadal status and the prominence of spawning tubercles were investigated. Nine riverine sites were investigated on the Elbe that were influenced by different sources of endocrine-active substances. Bream were collected from a lake that received no domestic or industrial discharges as a control. Plasma VTG concentrations were significantly higher in male bream from the Czech border to the middle Elbe, with the highest concentrations in fish sampled at the locations near Magdeburg and downstream of Dresden (between 20 and 100 times higher than in the controls), regions that are characterized by high levels of effluent discharges into the river. Following the Elbe from this site to the sea, the concentrations of plasma VTG in males were lower than at Meissen but were still elevated above the controls. 11KT and E2 titers showed suppressions in their normal concentrations at some locations (those receiving the greatest industrial discharges). There were reciprocal relationships between inhibitory effects on gonadal growth, maturation, and plasma sex steroids and exposure to pollutants, such as organotins, pesticides, or metals. However, there was no single chemical that alone could explain the observed inhibitory effects on sexual development. The results indicate that the endocrine system in wild bream is disrupted in stretches of the Elbe River.